Freitas Anna V, Herb Jake T, Pan Miao, Chen Yong, Gucek Marjan, Jin Tian, Xu Hong
National Heart, Lung and Blood Institute, National Institute of Health, Bethesda, MD 20892, USA.
National Institute of Allergy and Infectious Diseases, National Institute of Health, Rockville, MD 20852, USA.
iScience. 2022 Apr 29;25(5):104332. doi: 10.1016/j.isci.2022.104332. eCollection 2022 May 20.
The social ameba has emerged as a powerful model to study mitochondrial genetics and bioenergetics. However, a comprehensive inventory of mitochondrial proteins that is critical to understanding mitochondrial processes has yet to be curated. Here, we utilized high-throughput multiplexed protein quantitation and homology analyses to generate a high-confidence mitochondrial protein compendium consisting of 936 proteins. Our proteomic approach, which utilizes mass spectrometry in combination with mathematical modeling, was validated through mitochondrial targeting sequence prediction and live-cell imaging. Our final compendium consists of 936 proteins. Nearly, a third of mitochondrial proteins do not have homologs in humans, budding yeasts, or an ancestral alphaproteobacteria. Additionally, we leverage our compendium to highlight the complexity of metabolic reprogramming during starvation-induced development. Our compendium lays a foundation to investigate mitochondrial processes that are unique in ameba and to understand the functions of conserved mitochondrial proteins in .
社会性变形虫已成为研究线粒体遗传学和生物能量学的强大模型。然而，对于理解线粒体过程至关重要的线粒体蛋白质的全面清单尚未整理出来。在这里，我们利用高通量多重蛋白质定量和同源性分析，生成了一个由936种蛋白质组成的高可信度线粒体蛋白质汇编。我们的蛋白质组学方法结合了质谱和数学建模，并通过线粒体靶向序列预测和活细胞成像进行了验证。我们最终的汇编包含936种蛋白质。近三分之一的线粒体蛋白质在人类、芽殖酵母或祖先α-变形菌中没有同源物。此外，我们利用我们的汇编来突出饥饿诱导发育过程中代谢重编程的复杂性。我们的汇编为研究变形虫中独特的线粒体过程以及理解保守线粒体蛋白质在其中的功能奠定了基础。
