Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Howard Hughes Medical Institute and Departments of Molecular Biology and Medicine, Massachusetts General Hospital, Boston, MA 02114, USA.
Nucleic Acids Res. 2021 Jan 8;49(D1):D1541-D1547. doi: 10.1093/nar/gkaa1011.
The mammalian mitochondrial proteome is under dual genomic control, with 99% of proteins encoded by the nuclear genome and 13 originating from the mitochondrial DNA (mtDNA). We previously developed MitoCarta, a catalogue of over 1000 genes encoding the mammalian mitochondrial proteome. This catalogue was compiled using a Bayesian integration of multiple sequence features and experimental datasets, notably protein mass spectrometry of mitochondria isolated from fourteen murine tissues. Here, we introduce MitoCarta3.0. Beginning with the MitoCarta2.0 inventory, we performed manual review to remove 100 genes and introduce 78 additional genes, arriving at an updated inventory of 1136 human genes. We now include manually curated annotations of sub-mitochondrial localization (matrix, inner membrane, intermembrane space, outer membrane) as well as assignment to 149 hierarchical 'MitoPathways' spanning seven broad functional categories relevant to mitochondria. MitoCarta3.0, including sub-mitochondrial localization and MitoPathway annotations, is freely available at http://www.broadinstitute.org/mitocarta and should serve as a continued community resource for mitochondrial biology and medicine.
哺乳动物的线粒体蛋白质组受双重基因组控制，其中 99%的蛋白质由核基因组编码，13 种来自线粒体 DNA（mtDNA）。我们之前开发了 MitoCarta，这是一个包含超过 1000 种编码哺乳动物线粒体蛋白质组的基因目录。该目录是通过对来自 14 种鼠组织分离的线粒体进行蛋白质质谱分析，结合多种序列特征和实验数据集的贝叶斯综合方法编制而成的。在这里，我们介绍 MitoCarta3.0。从 MitoCarta2.0 清单开始，我们进行了手动审查，删除了 100 个基因，并引入了另外 78 个基因，最终更新了 1136 个人类基因的清单。我们现在包括了对亚线粒体定位（基质、内膜、膜间空间、外膜）的手动注释，以及对跨越与线粒体相关的七个广泛功能类别的 149 个层次“MitoPathways”的分配。MitoCarta3.0 包括亚线粒体定位和 MitoPathway 注释，可在 http://www.broadinstitute.org/mitocarta 免费获得，它应该作为线粒体生物学和医学的持续社区资源。
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