新秀丽根瘤菌（Neorhizobium xiangyangii），从西藏昌都高原青稞种植土壤中分离得到。

Neorhizobium xiangyangii sp. nov., isolated from a highland barley cultivation soil in Qamdo, Tibet.

机构信息

College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, China.

Institute of Agricultural Product Quality Standard and Testing Research, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, 850032, China.

出版信息

Arch Microbiol. 2022 May 23;204(6):345. doi: 10.1007/s00203-022-02961-3.

DOI:10.1007/s00203-022-02961-3

PMID:35604458

Abstract

A novel Gram-negative, aerobic, rod-shaped and non-nitrogen fixing bacterium named T786 was isolated from a highland barley cultivation soil in Qamdo, Tibet Autonomous Region, PR China. Strain T786 grew at 5-30 ℃ and pH 6.0-10.0 (optimum, 20-25 ℃ and pH 7.0-8.0) with 0-4% (w/v) NaCl (optimum, 0%). The 16S rRNA gene sequences of strain T786 showed the highest similarity to Neorhizobium vignae CCBAU 05176 (98.7%), followed by Neorhizobium alkalisoli CCBAU 01393 (98.5%), Neorhizobium tomejilense T17_20 (98.4%), Neorhizobium huautlense S02 (98.4%), and Neorhizobium galegae ATCC 43677 (98.0%). Phylogenetic analysis based on 16S rRNA genes indicated that strain T786 was a new member of the genus Neorhizobium. The digital DNA-DNA hybridization and average nucleotide identity values between strain T786 and related strains were estimated as 20.2-20.6% and 76.6-80.0%, respectively. The genomic DNA G + C content based on the draft genome sequence was 60.2%. The major cellular fatty acids were Summed feature 8 (C ω7c or C ω6c), C and Summed feature 3 (C ω7c or C ω6c). The polar lipids were diphosphatidyl glycerol, phosphatidyl glycerol, phosphatidyl ethanolamine, phosphatidyl methyl ethanolamine, unidentified phospholipid and unidentified lipids (1-4). The isoprenoid quinone was ubiquinone-10. The DAP and sugar components of cell wall were meso-DAP and ribose, glucose, respectively. Based on phenotypic, phylogenetic, and genotypic data, for which the name Neorhizobium xiangyangii sp. nov. is proposed. The type strain is T786 (= JCM 35100 = CICC 25102).

摘要

一株新型革兰氏阴性、需氧、杆状、不固氮的细菌，命名为 T786，从中国西藏自治区的一个青稞农田土壤中分离出来。T786 菌株在 5-30°C 和 pH6.0-10.0（最佳条件为 20-25°C 和 pH7.0-8.0），能耐受 0-4%（w/v）的 NaCl（最佳条件为 0%）。菌株 T786 的 16S rRNA 基因序列与 Neorhizobium vignae CCBAU 05176（98.7%）最为相似，其次是 Neorhizobium alkalisoli CCBAU 01393（98.5%）、Neorhizobium tomejilense T17_20（98.4%）、Neorhizobium huautlense S02（98.4%）和 Neorhizobium galegae ATCC 43677（98.0%）。基于 16S rRNA 基因的系统发育分析表明，T786 菌株是 Neorhizobium 属的一个新成员。T786 菌株与相关菌株的数字 DNA-DNA 杂交和平均核苷酸同源性值分别估计为 20.2-20.6%和 76.6-80.0%。根据基因组草图序列计算的基因组 DNA G+C 含量为 60.2%。主要的细胞脂肪酸为 Summed feature 8（C ω7c 或 C ω6c）、C 和 Summed feature 3（C ω7c 或 C ω6c）。极性脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甲基乙醇胺、未鉴定的磷脂和未鉴定的脂类（1-4）。异戊二烯醌为泛醌-10。细胞壁的 DAP 和糖成分分别为 meso-DAP 和核糖、葡萄糖。基于表型、系统发育和基因型数据，提出了 Neorhizobium xiangyangii sp. nov. 的新名称。模式菌株为 T786（=JCM 35100=CICC 25102）。