Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.
Artificial Intelligence and Multi-Omics Center (AIMOC), Stavanger, Norway.
Sci Rep. 2022 May 23;12(1):8676. doi: 10.1038/s41598-022-12757-6.
Acanthamoeba polyphaga mimivirus (APMV), a species of amoeba-infecting giant viruses, has recently emerged as human respiratory pathogens. This study aimed to evaluate the presence of Mimivirus in respiratory samples, collected from tuberculosis (TB)-suspected patients. The study was performed on 10,166 clinical respiratory samples from April 2013 to December 2017. Mimivirus was detected using a suicide nested-polymerase chain reaction (PCR) and real-time PCR methods. Of 10,166 TB-suspected patients, 4 (0.04%) were positive for Mimivirus, including Mimivirus-53, Mimivirus-186, Mimivirus-1291, and Mimivirus-1922. Three out of four patients, hospitalized in the intensive care unit (ICU), were mechanically ventilated. All patients had an underlying disease, and the virus was detected in both sputum and bronchoalveolar lavage samples. In conclusion, Mimivirus was isolated from TB-suspected patients in a comprehensive study. The present results, similar to previous reports, showed that Mimiviruses could be related to pneumonia. Further studies in different parts of the world are needed to additional investigate the clinical importance of Mimivirus infection.
噬棘阿米巴 polyphaga mimic 病毒（APMV），一种感染变形虫的巨型病毒，最近已成为人类呼吸道病原体。本研究旨在评估从疑似结核病（TB）患者的呼吸道样本中是否存在 Mimivirus。该研究于 2013 年 4 月至 2017 年 12 月对 10166 例临床呼吸道样本进行了研究。使用自杀嵌套聚合酶链反应（PCR）和实时 PCR 方法检测 Mimivirus。在 10166 例疑似结核病患者中，有 4 例（0.04%）Mimivirus 阳性，包括 Mimivirus-53、Mimivirus-186、Mimivirus-1291 和 Mimivirus-1922。4 例患者中有 3 例（75%）住院于重症监护病房（ICU），均接受机械通气。所有患者均患有基础疾病，病毒在痰和支气管肺泡灌洗液样本中均被检出。总之，本综合研究从疑似结核病患者中分离出 Mimivirus。与以往报道相似，本研究结果表明 Mimiviruses 可能与肺炎有关。需要在世界不同地区开展进一步研究，以进一步探讨 Mimivirus 感染的临床重要性。
