Lopes M T, Liberato M H, Widman A, Brentani M M
J Steroid Biochem. 1987 Feb;26(2):219-26. doi: 10.1016/0022-4731(87)90075-6.
Using a saturation analysis over a wide range of [3H]estradiol at two temperatures 4 and 22 degrees C we have determined unoccupied (4 degrees C) and total (22 degrees C) type II estrogen binding site (EBS) levels in individual cytosols of 100 patients with breast cancer (50 post and 50 premenopausal). Exchange was found to be complete after 18 h at 22 degrees C and receptor degradation was negligible during this treatment. Steroid specificity and affinity determined by Scatchard and Rosenthal plot analysis were not altered at 22 degrees C. Carcinomas presented a higher total type II REBS level as compared unfilled type II binding sites or the classical ER, independently of menopausal status, phase of the menstrual cycle or positivity of ER. On the other hand, unoccupied type II EBS level was strongly correlated to the concentration of type I ER, being higher on the post-menopausal group and older patients.
在4℃和22℃两个温度下，对一系列广泛浓度的[3H]雌二醇进行饱和分析，我们测定了100例乳腺癌患者（50例绝经后和50例绝经前）个体胞质溶胶中未占据（4℃）和总（22℃）II型雌激素结合位点（EBS）水平。发现在22℃下18小时后交换完全，并且在此处理过程中受体降解可忽略不计。通过Scatchard和Rosenthal作图分析确定的类固醇特异性和亲和力在22℃下未改变。无论绝经状态、月经周期阶段或ER阳性情况如何，癌组织中总II型REBS水平均高于未填充的II型结合位点或经典ER。另一方面，未占据的II型EBS水平与I型ER浓度密切相关，在绝经后组和老年患者中更高。
