Perera D M, McGarrigle H H, Lawrence D M, Lucas M
J Steroid Biochem. 1987 Feb;26(2):273-7. doi: 10.1016/0022-4731(87)90082-3.
Unconjugated testosterone levels were assayed in 351 amniotic fluid samples obtained at 15-19 weeks gestation. The median values for unconjugated testosterone in the 166 female foetuses and 185 male foetuses were 137 and 712 pmol/l respectively. Sixteen amniotic fluid samples from male foetuses had unconjugated testosterone levels lower than the highest female unconjugated testosterone value (361 pmol/l). Testosterone glucuronide was measured in amniotic fluid from 48 female and 55 male foetuses. There was a significant sex difference in the median values of testosterone glucuronide between female (median 160 pmol/l, range 64-465 pmol/l) and male (median 817 pmol/l, range 68-3707 pmol/l) amniotic fluid specimens (P less than 0.001). Of the sixteen male foetuses with amniotic fluid unconjugated testosterone levels in the female range, 12 had amniotic fluid testosterone glucuronide levels within the male testosterone glucuronide range of values. Hence used in conjunction with unconjugated testosterone, testosterone glucuronide increased the predictive accuracy of foetal sexing from 95.4 to 98.9%. Testosterone sulphate was measured in 24 female and 25 male amniotic fluid samples. There was no Testosterone sulphate was measured in 24 female and 25 male amniotic fluid samples. There was no significant difference between female (median 2591 pmol/l) and male (median 2964 pmol/l) testosterone sulphate levels.
对妊娠15至19周时获取的351份羊水样本进行了未结合睾酮水平检测。166例女胎和185例男胎羊水样本中未结合睾酮的中位数分别为137和712 pmol/l。16份来自男胎的羊水样本未结合睾酮水平低于女胎未结合睾酮的最高值（361 pmol/l）。对48例女胎和55例男胎的羊水进行了睾酮葡萄糖醛酸苷检测。女胎（中位数160 pmol/l，范围64 - 465 pmol/l）和男胎（中位数817 pmol/l，范围68 - 3707 pmol/l）羊水样本中睾酮葡萄糖醛酸苷的中位数存在显著性别差异（P < 0.001）。在羊水未结合睾酮水平处于女性范围内的16例男胎中，有12例的羊水睾酮葡萄糖醛酸苷水平处于男性睾酮葡萄糖醛酸苷值范围内。因此，与未结合睾酮联合使用时，睾酮葡萄糖醛酸苷将胎儿性别鉴定的预测准确率从95.4%提高到了98.9%。对24份女性和25份男性羊水样本进行了硫酸睾酮检测。女性（中位数2591 pmol/l）和男性（中位数2964 pmol/l）的硫酸睾酮水平无显著差异。
