Robertson R D, Henniker A J, Luttrell B M, Saunders D M
Eur J Obstet Gynecol Reprod Biol. 1980 Feb;10(2):77-81. doi: 10.1016/0028-2243(80)90085-4.
Amniotic fluid testosterone levels were measured by radioimmunoassay without chromatography on 101 specimens obtained at amniocenteses between 15 and 19 wk gestation. For the male fetus, the amniotic fluid testosterone level of 553 +/- 23 pmol/l (mean +/- SE) was significantly higher (P less than 0.0005) than the concentration found for the female fetus (206 +/- 9 pmol/l). There was an overlap of the ranges 74-1120 pmol/l for the male and 122-399 pmol/l for the female fetuses. Amniotic fluid testosterone levels above 400 pmol/l were observed in 84% of the male and in none of the female fetuses. The method allowed determination of testosterone levels within 8 h. It is concluded that amniotic fluid testosterone measured by radioimmunoassay without chromatography is a rapid and effective preliminary screening test for the prenatal diagnosis of fetal sex.
采用放射免疫分析法，对妊娠15至19周期间羊膜穿刺获得的101份样本进行非色谱法检测羊水睾酮水平。对于男性胎儿，羊水睾酮水平为553±23 pmol/l（均值±标准误），显著高于女性胎儿的浓度（206±9 pmol/l）（P<0.0005）。男性胎儿的范围为74 - 1120 pmol/l，女性胎儿的范围为122 - 399 pmol/l，二者存在重叠。在84%的男性胎儿中观察到羊水睾酮水平高于400 pmol/l，而女性胎儿中无一例出现这种情况。该方法可在8小时内测定睾酮水平。结论是，采用非色谱法的放射免疫分析法测定羊水睾酮是一种快速有效的胎儿性别产前诊断初步筛查试验。
