Trace Element, Spectroscopy and Speciation Group (GETEE), Instituto de Materiais (iMATUS), Faculty of Chemistry, University of Santiago de Compostela, Av. das Ciencias s/n, 15782, Santiago de Compostela, Spain.
Trace Element, Spectroscopy and Speciation Group (GETEE), Instituto de Materiais (iMATUS), Faculty of Chemistry, University of Santiago de Compostela, Av. das Ciencias s/n, 15782, Santiago de Compostela, Spain.
Talanta. 2022 Sep 1;247:123556. doi: 10.1016/j.talanta.2022.123556. Epub 2022 May 18.
Seaweed can bioaccumulate nanomaterials that would be transferred to the trophic chain. This work describes the optimization of a method for the separation of silver nanoparticles (AgNPs) from seaweed using an ultrasound-assisted enzymatic hydrolysis method and ulterior determination by single particle inductively coupled plasma mass spectrometry (SP-ICP-MS). The following parameters affecting the isolation of AgNPs were optimized using a Palmaria palmata (red seaweed) sample previously exposed to AgNPs: type of sonication (bath vs. ultrasonic probe), ultrasound amplitude, sonication time, sonication mode (pulsed vs. continuous sonication), concentration of the enzymes mixture (Macerozyme R-10®), and enzymatic hydrolysis time. The stability of AgNPs during extraction was tested by transmission electron microscopy (TEM) and using a standard of 15 nm of polyvinylpyrrolidone (PVP)-coated AgNPs analyzed by SP-ICP-MS. The analytical performance was evaluated with good results. For total Ag determination, the limits of detection and quantification were 2.2 and 7.7 ng g, respectively; and for AgNPs determination, the limits of detection in size and number were 14 nm and 4.34 × 10 part g, respectively. Besides, the matrix effect, the repeatability and the analytical recovery were also studied. Finally, the method was applied to the analysis of several red (Palmaria palmata) and green (Ulva sp.) seaweed samples.
海藻可以生物累积纳米材料，这些材料将被转移到食物链中。本工作描述了一种使用超声辅助酶解方法从海藻中分离银纳米粒子（AgNPs）的方法的优化，并通过单颗粒电感耦合等离子体质谱（SP-ICP-MS）进行了进一步的测定。使用先前暴露于 AgNPs 的掌状红皮藻（Palmaria palmata）样品优化了影响 AgNPs 分离的以下参数：超声类型（浴式与超声探头）、超声幅度、超声时间、超声模式（脉冲与连续超声）、酶混合物浓度（Macerozyme R-10®）和酶解时间。通过透射电子显微镜（TEM）和使用标准的 15nm 聚维酮（PVP）包覆的 AgNPs 进行 SP-ICP-MS 分析来测试 AgNPs 在提取过程中的稳定性。用该方法对总 Ag 进行测定，检出限和定量限分别为 2.2 和 7.7ng g；对 AgNPs 进行测定时，粒径和数量的检出限分别为 14nm 和 4.34×10 个颗粒 g。此外，还研究了基质效应、重复性和分析回收率。最后，该方法应用于几种红藻（掌状红皮藻）和绿藻（Ulvasp.）海藻样品的分析。
