Department of Biology, University of Rochester, Rochester, New York, USA; email:
Center for RNA Biology and Department of Biochemistry and Biophysics, University of Rochester, Rochester, New York, USA.
Annu Rev Microbiol. 2022 Sep 8;76:193-210. doi: 10.1146/annurev-micro-041020-121806. Epub 2022 May 24.
The pace and efficiency of ribosomal subunit production directly impact the fitness of bacteria. Biogenesis demands more than just the union of ribosomal components, including RNA and proteins, to form this functional ribonucleoprotein particle. Extra-ribosomal protein factors play a fundamental role in the efficiency and efficacy of ribosomal subunit biogenesis. A paucity of data on intermediate steps, multiple and overlapping pathways, and the puzzling number of functions that extra-ribosomal proteins appear to play in vivo make unraveling the formation of this macromolecular assemblage difficult. In this review, we outline with examples the multinodal landscape of factor-assisted mechanisms that influence ribosome synthesis in bacteria. We discuss in detail late-stage events that mediate correct ribosome formation and the transition to translation initiation and thereby ensure high-fidelity protein synthesis.
核糖体亚基的产生速度和效率直接影响细菌的适应性。生物发生不仅需要核糖体成分（包括 RNA 和蛋白质）的结合，以形成这种功能性核糖核蛋白颗粒。额外的核糖体蛋白因子在核糖体亚基生物发生的效率和效果中起着至关重要的作用。关于中间步骤、多种重叠途径以及额外核糖体蛋白在体内似乎发挥的令人困惑的众多功能的数据很少，这使得解开这种大分子组装体的形成变得困难。在这篇综述中，我们通过实例概述了影响细菌中核糖体合成的因子辅助机制的多节点景观。我们详细讨论了介导正确核糖体形成和向翻译起始的转变的晚期事件，从而确保了高保真的蛋白质合成。
