Nagler A, Riklis I, Tatarsky I, Fabian I
Leuk Res. 1987;11(4):379-84. doi: 10.1016/0145-2126(87)90184-6.
The effect of low dose (10(-12)-10(-7) M) ARA-C on differentiation and proliferation in liquid and semisolid culture of marrow cells from 13 patients with myelodysplastic syndrome (MDS) were studied following incubation in liquid culture with low dose ARA-C. In six of ten patients an increasing number of myeloid cells acquired the morphologic appearance of mature monocyte-macrophages. Increasing number of cells reacted positively to fluoride sensitive naphthyl acetate esterase and specifically bound MY4 monoclonal antibody. Phagocytosis and killing of Candida albicans by monocyte-macrophages incubated with low dose ARA-C was normal and similar to that of the untreated cells. All MDS patients showed reduced myeloid colony and increased cluster formation. Low dose ARA-C had slight but non-significant inhibitory effects on myeloid colony growth. The results indicated that the differentiation pattern of myeloid precursor cells form a subset of MDS patients was altered by exposure to low dose ARA-C in vitro.
研究了低剂量（10⁻¹² - 10⁻⁷M）阿糖胞苷（ARA-C）对13例骨髓增生异常综合征（MDS）患者骨髓细胞在液体和半固体培养中分化和增殖的影响，这些患者的骨髓细胞在液体培养中与低剂量ARA-C一起孵育。在10例患者中的6例中，越来越多的髓系细胞获得了成熟单核细胞-巨噬细胞的形态外观。越来越多的细胞对氟化物敏感的萘乙酸酯酶呈阳性反应，并特异性结合MY4单克隆抗体。与低剂量ARA-C一起孵育的单核细胞-巨噬细胞对白色念珠菌的吞噬和杀伤作用正常，与未处理细胞相似。所有MDS患者的髓系集落减少，集簇形成增加。低剂量ARA-C对髓系集落生长有轻微但不显著的抑制作用。结果表明，体外暴露于低剂量ARA-C会改变MDS患者亚组中髓系前体细胞的分化模式。
