Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, UK.
Department of Genetics and Genome Biology, University of Leicester, Leicester, UK.
Methods Mol Biol. 2022;2482:385-394. doi: 10.1007/978-1-0716-2249-0_26.
The use of nonclassical model organisms for biological rhythm research has become popular in the last two decades. Here we describe techniques for delivery of dsRNAi molecules to knock down clock gene transcripts in a small intertidal crustacean, Eurydice pulchra, as well as our method for immunodetection of clock proteins in the brain. These methods can be generalized for gene knockdown in any small crustacean or arthropod in which mutagenesis by other methods is neither practical nor possible.
在过去的二十年中，非典型模式生物在生物节律研究中的应用变得越来越流行。在这里，我们描述了向小型潮间带甲壳动物 Eurydice pulchra 中递送 dsRNAi 分子以敲低生物钟基因转录本的技术，以及我们在脑中检测生物钟蛋白的免疫检测方法。这些方法可以推广到任何其他方法都不实际或不可能进行诱变的小型甲壳动物或节肢动物中的基因敲低。
