Harnpicharnchai P, Mayteeworakoon S, Kitikhun S, Chunhametha S, Likhitrattanapisal S, Eurwilaichitr L, Ingsriswang S
National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pahonyothin Road, Pathum Thani, Thailand.
National Science and Technology Development Agency, Phahonyothin Road, Pathum Thani, Thailand.
Lett Appl Microbiol. 2022 Oct;75(4):888-898. doi: 10.1111/lam.13748. Epub 2022 Jun 23.
This study demonstrates a remarkably high level of microbial-induced calcium carbonate precipitation (MICP) using a mixed culture containing TBRC 1396 (Priestia megaterium), TBRC 8147 (Neobacillus drentensis) and ATCC 11859 (Sporosarcina pasteurii) bacterial strains. The mixed culture produced CaCO weights 1·4 times higher than those obtained from S. pasteurii, the gold standard for efficient MICP processes. The three strains were selected after characterization of various Bacillus spp. and related species for their ability to induce the MICP process, especially in an alkaline and high-temperature environment. Results showed that the TBRC 1396 and TBRC 8147 strains, as well as TBRC 5949 (Bacillus subtilis) and TBRC 8986 (Priestia aryabhattai) strains, could generate calcium carbonate at pH 9-12 and temperature 30-40°C, which is suitable for construction and consolidation purposes. The TBRC 8147 strain also exhibited CaCO precipitation at 45°C. The TBRC 8986 and TBRC 8147 strains are nonureolytic bacteria capable of MICP in the absence of urea, which can be used to avoid the generation of undesirable ammonia associated with the ureolytic MICP process. These findings facilitate the successful use of MICP as a sustainable and environmentally friendly technology for the development of various materials, including self-healing concrete and soil consolidation.
本研究表明，使用含有泰国培养物保藏中心1396（巨大Priestia菌）、泰国培养物保藏中心8147（德伦特新芽孢杆菌）和美国典型培养物保藏中心11859（巴斯德芽孢八叠球菌）菌株的混合培养物，微生物诱导碳酸钙沉淀（MICP）水平显著较高。该混合培养物产生的碳酸钙重量比高效MICP过程的金标准——巴斯德芽孢八叠球菌所产生的碳酸钙重量高1.4倍。在对各种芽孢杆菌属和相关菌种进行表征以确定它们诱导MICP过程的能力后，选择了这三种菌株，尤其是在碱性和高温环境中的诱导能力。结果表明，泰国培养物保藏中心1396和泰国培养物保藏中心8147菌株，以及泰国培养物保藏中心5949（枯草芽孢杆菌）和泰国培养物保藏中心8986（阿耶波多Priestia菌）菌株，能够在pH值为9 - 12、温度为30 - 40°C的条件下生成碳酸钙，这适用于建筑和加固目的。泰国培养物保藏中心8147菌株在45°C时也表现出碳酸钙沉淀。泰国培养物保藏中心8986和泰国培养物保藏中心8147菌株是无脲酶细菌，能够在没有尿素的情况下进行MICP，这可用于避免与脲酶MICP过程相关的不良氨的产生。这些发现有助于成功地将MICP作为一种可持续且环保的技术用于开发各种材料，包括自愈合混凝土和土壤加固。
