College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
J Mater Chem B. 2022 Jun 15;10(23):4473-4478. doi: 10.1039/d2tb00435f.
With the prevalence of diabetes, rapid and simple blood glucose monitoring has become more and more important. Here, we report the synthesis of bio-templated N-CdZnTeS quantum dots (QDs), which are great fluorescent biological labels and were used for the fabrication of dual-emissive dye@protein-QD conjugates copper-free click chemistry, such as the 5(6)-carboxyfluorescein@glucose oxidase-quantum dot (FAM@GOx-QDs) complex. When adding glucose, the red fluorescence of the CdZnTeS QDs sharply decreased, while the green fluorescence of FAM was invariable. A good linear relationship ranging from 0.3 to 30 μM was obtained for glucose detection, with the limit of detection as low as 0.035 μM. Notably, the DNA-bridging FAM@GOx-QDs complex exhibited enhanced enzyme activity and stability, and was applied for the differentiation of diabetic and healthy people by the naked eye.
随着糖尿病的流行，快速简便的血糖监测变得越来越重要。在这里，我们报告了生物模板 N-CdZnTeS 量子点（QDs）的合成，这些量子点是出色的荧光生物标记物，并用于制备双发射染料@蛋白质-QD 缀合物，例如 5（6）-羧基荧光素@葡萄糖氧化酶-QD（FAM@GOx-QDs）复合物。当添加葡萄糖时，CdZnTeS QDs 的红色荧光急剧下降，而 FAM 的绿色荧光保持不变。对于葡萄糖检测，获得了从 0.3 到 30 μM 的良好线性关系，检测限低至 0.035 μM。值得注意的是，DNA 桥接的 FAM@GOx-QDs 复合物表现出增强的酶活性和稳定性，并通过肉眼应用于糖尿病患者和健康人的区分。
