Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University , Wuhan 430072, China.
Department of Laboratory Medicine, Zhongnan Hospital, Wuhan University , Wuhan 430071, China.
Anal Chem. 2017 Nov 7;89(21):11628-11635. doi: 10.1021/acs.analchem.7b03053. Epub 2017 Oct 26.
As the blood glucose concentration is an important clinical parameter of diabetes, the rapid and effective detection of blood glucose is very significant for monitoring and managing diabetes. Here, a facile method to prepare Rox-DNA functionalized CdZnTeS quantum dots (QDs) was developed. The Rox-DNA functionalized CdZnTeS QDs were prepared by a one-pot hydrothermal method through phosphorothioate DNA bound to QDs, which were employed as a ratiometric fluorescent probe for the rapid and sensitive detection of HO and glucose. Compared with the traditional multistep construction of ratiometric fluorescent probes, this presented approach is simpler and more effective without chemical modification and complicated separation. The CdZnTeS QDs with green fluorescence is specifically sensitive to HO, while the red fluorescence of Rox is invariable. HO is the product from the oxidation of glucose catalyzed by glucose oxidase (GOx). Therefore, a facile method to detect HO and glucose with a detection limit of 0.075 μM for HO and 0.042 μM for glucose was developed. In addition, this proposed probe has been employed for the detection of glucose in human serum with a satisfactory result. Moreover, this probe has been used for visual detection, and the health and diabetics can be distinguished by the naked eye. Meanwhile, this nanoprobe is also generalizable and can be extended to the detection of many other HO-mediated analytes.
由于血糖浓度是糖尿病的一个重要临床参数，因此快速有效地检测血糖对于监测和管理糖尿病非常重要。在这里，开发了一种简便的方法来制备 Rox-DNA 功能化的 CdZnTeS 量子点（QDs）。Rox-DNA 功能化的 CdZnTeS QDs 通过磷硫代 DNA 结合 QDs 一步水热法制备，可用作比率荧光探针，用于快速灵敏地检测 HO 和葡萄糖。与传统的比率荧光探针的多步构建相比，该方法更简单、更有效，无需化学修饰和复杂分离。具有绿色荧光的 CdZnTeS QDs 对 HO 具有特异性敏感性，而 Rox 的红色荧光则保持不变。HO 是葡萄糖氧化酶（GOx）催化葡萄糖氧化的产物。因此，开发了一种简单的方法来检测 HO 和葡萄糖，其对 HO 的检测限为 0.075 μM，对葡萄糖的检测限为 0.042 μM。此外，该探针已用于人血清中葡萄糖的检测，结果令人满意。此外，该探针还可用于可视化检测，通过肉眼可以区分健康人和糖尿病患者。同时，这种纳米探针具有通用性，可以扩展到检测许多其他由 HO 介导的分析物。
