Bocharova Yu A, Savinova T A, Lyamin A V, Kondratenko O V, Polikarpova S V, Zhilina S V, Fedorova N I, Semykin S Yu, Chaplin A V, Korostin D O, Mayansky N A, Chebotar I V
Pirogov Russian National Research Medical University.
Samara State Medical University.
Klin Lab Diagn. 2022 May 21;67(5):315-320. doi: 10.51620/0869-2084-2022-67-5-315-320.
Stenotrophomonas maltophilia is a common opportunistic microorganism and an important respiratory pathogen in cystic fibrosis (CF). The aim of this study was to determine antimicrobial resistance phenotypes, sequence-types (ST) and genetic determinants of antibiotic resistance in S. maltophilia strains recovered from CF patients in Russia. S. maltophilia isolates recovered from 170 CF patients were analyzed. Minimum inhibitory concentrations of antibacterial agents were determined using Sensititre Gram Negative GNX2F plates and the results were interpreted according to Clinical and Laboratory Standards Institute (CLSI) criteria. Whole-genome sequencing (WGS) was performed on MGISEQ-2000 platform. SPAdes software, Galaxy, ResFinder, Integrall and PubMLST were used for analysis of WGS data. S. maltophilia strains were identified from 24/170 (14%) CF patients. In total, 25 isolates were detected, two strains were isolated from the same patient. The isolates belonged to 17 different STs, including 5 new STs; ST4 was the most prevalent ST. Resistance to ceftazidime was observed in 60% of strains, to ticarcillin-clavulanate - in 32%, to levofloxacin - in 24%, to trimethoprim/sulfamethoxazole - in 12% of strains. All isolates were susceptible to minocycline. All ST4 isolates were resistant or intermediate to ceftazidime and ticarcillin-clavulanate. In two isolates, the sul1 gene was detected. In one isolate, sul1 was part of a class 1 integron. The detected integron also contained the blaGES-7 and aac(6')-Ib-cr genes. The ST4 sequence-type was the most prevalent ST among S. maltophilia strains recovered from CF patients in Russia. Antibiotic resistance genes, including sul1, blaGES-7, aac(6')-Ib-cr, were detected in single strains.
嗜麦芽窄食单胞菌是一种常见的机会性微生物，也是囊性纤维化（CF）患者重要的呼吸道病原体。本研究旨在确定从俄罗斯CF患者中分离出的嗜麦芽窄食单胞菌菌株的抗菌药物耐药表型、序列型（ST）及抗生素耐药的遗传决定因素。对从170例CF患者中分离出的嗜麦芽窄食单胞菌进行了分析。使用Sensititre革兰氏阴性GNX2F平板测定抗菌药物的最低抑菌浓度，并根据临床和实验室标准协会（CLSI）标准解释结果。在MGISEQ - 2000平台上进行全基因组测序（WGS）。使用SPAdes软件、Galaxy、ResFinder、Integrall和PubMLST对WGS数据进行分析。从24/170（14%）例CF患者中鉴定出嗜麦芽窄食单胞菌菌株。共检测到25株分离菌，有两株来自同一患者。这些分离菌属于17种不同的ST，包括5种新的ST；ST4是最常见的ST。60%的菌株对头孢他啶耐药，32%对替卡西林 - 克拉维酸耐药，24%对左氧氟沙星耐药，12%的菌株对甲氧苄啶/磺胺甲恶唑耐药。所有分离菌对米诺环素敏感。所有ST4分离菌对头孢他啶和替卡西林 - 克拉维酸耐药或中介。在两株分离菌中检测到sul1基因。在一株分离菌中，sul1是1类整合子的一部分。检测到的整合子还包含blaGES - 7和aac(6') - Ib - cr基因。ST4序列型是从俄罗斯CF患者中分离出的嗜麦芽窄食单胞菌菌株中最常见的ST。在单株菌株中检测到了包括sul1、blaGES - 7、aac(6') - Ib - cr在内的抗生素耐药基因。
