Pirogov Russian National Research Medical University.
Samara State Medical University.
Klin Lab Diagn. 2021 Oct 18;66(10):629-634. doi: 10.51620/0869-2084-2021-66-10-629-634.
Cystic fibrosis (CF) is a common genetic disease, manifested by airway obstruction and chronic respiratory infection. The most prevalent infectious agent in airways of CF patients is Pseudomonas aeruginosa. This study aimed to determine sequence-types, antimicrobial resistance phenotypes and genes defining adaptive antibiotic resistance in P. aeruginosa isolates recovered from CF patients in Russia. In total, 84 P. aeruginosa strains from 64 CF patients were analyzed. Susceptibility to antibiotics was determined by disk diffusion test. Whole-genome sequencing (WGS) was performed on MGISEQ-2000 platform. SPAdes software, Galaxy, ResFinder, PubMLST were used for analysis of WGS data. Examined P. aeruginosa isolates belonged to 53 different sequence-types (STs), including 6 new STs. High-risk epidemic clone ST235 (10%) and clonal CF P. aeruginosa strains ST17, ST242, ST274 (7%) were detected. Non-susceptibility to ticarcillin-clavulanate, cefepime, imipenem was observed in 63%, 12% and 25% of isolates, respectively; to tobramycin - in 24%, to amikacin - in 35%; to ciprofloxacin, levofloxacin - in 35% and 57% of strains, respectively. Multidrug-resistant phenotype was detected in 18% of isolates. In examined strains, genes of beta-lactamases VIM-2 (5 ST235 strains), VEB-1 (two ST2592 strains), GES-1 (1 ST235 strain), PER-1 (1 ST235 strain) were found. Ciprofloxacin-modifying enzyme CrpP gene was detected in 67% of isolates, aminoglycoside-modifying enzymes AAD, ANT, AAC genes - in 7%, 4%, 12% of strains, respectively. P. aeruginosa isolates from CF patients in Russia demonstrate a high clonal diversity, which is similar to other P. aeruginosa infections. The isolates of high-risk clone and clonal CF P. aeruginosa strains are detected.
囊性纤维化（CF）是一种常见的遗传疾病，表现为气道阻塞和慢性呼吸道感染。CF 患者气道中最常见的感染病原体是铜绿假单胞菌。本研究旨在确定从俄罗斯 CF 患者中分离的铜绿假单胞菌分离株的序列型、抗菌药物耐药表型和定义适应性抗生素耐药的基因。总共分析了来自 64 名 CF 患者的 84 株铜绿假单胞菌。通过纸片扩散试验测定抗生素敏感性。使用 MGISEQ-2000 平台进行全基因组测序（WGS）。使用 SPAdes 软件、Galaxy、ResFinder、PubMLST 分析 WGS 数据。所研究的铜绿假单胞菌分离株属于 53 个不同的序列型（ST），包括 6 个新的 ST。高风险流行克隆 ST235（10%）和克隆 CF 铜绿假单胞菌 ST17、ST242、ST274（7%）被检测到。63%、12%和 25%的分离株分别对替卡西林-克拉维酸、头孢吡肟、亚胺培南不敏感；24%的分离株对妥布霉素不敏感，35%的分离株对阿米卡星不敏感；35%和 57%的分离株对环丙沙星、左氧氟沙星不敏感。18%的分离株表现出多药耐药表型。在检测的菌株中，发现了 VIM-2 型（5 株 ST235 株）、VEB-1 型（2 株 ST2592 株）、GES-1 型（1 株 ST235 株）和 PER-1 型（1 株 ST235 株）β-内酰胺酶基因。在 67%的分离株中检测到环丙沙星修饰酶 CrpP 基因，在 7%、4%和 12%的分离株中分别检测到氨基糖苷类修饰酶 AAD、ANT、AAC 基因。来自俄罗斯 CF 患者的铜绿假单胞菌分离株表现出高度的克隆多样性，与其他铜绿假单胞菌感染相似。已检测到高危克隆和克隆 CF 铜绿假单胞菌分离株。
