Laboratory for Optics and Biosciences, CNRS UMR 7645, Inserm U1182, Institut Polytechnique de Paris, 91128 Palaiseau, France.
Center for Information Services and High Performance Computing, Technische Universität Dresden, Dresden, Germany.
Dev Cell. 2022 Jun 20;57(12):1529-1544.e5. doi: 10.1016/j.devcel.2022.05.001. Epub 2022 May 24.
Morphogenesis, wound healing, and some cancer metastases depend upon the migration of cell collectives that need to be guided to their destination as well as coordinated with other cell movements. During zebrafish gastrulation, the extension of the embryonic axis is led by the mesendodermal polster that migrates toward the animal pole, followed by the axial mesoderm that undergoes convergence and extension. Here, we investigate how polster cells are guided toward the animal pole. Using a combination of precise laser ablations, advanced transplants, and functional as well as in silico approaches, we establish that each polster cell is oriented by its immediate follower cells. Each cell perceives the migration of followers, through E-cadherin/α-catenin mechanotransduction, and aligns with them. Therefore, directional information propagates from cell to cell over the whole tissue. Such guidance of migrating cells by followers ensures long-range coordination of movements and developmental robustness.
形态发生、伤口愈合和一些癌症转移依赖于细胞集体的迁移，这些细胞需要被引导到目的地，并与其他细胞运动相协调。在斑马鱼原肠胚形成过程中，胚胎轴的延伸由向动物极迁移的中胚层间质引导，随后是轴向中胚层经历汇聚和延伸。在这里，我们研究了间质细胞是如何被引导到动物极的。我们使用精确的激光消融、先进的移植技术以及功能和计算机模拟方法的结合，确定每个间质细胞都被其直接的后续细胞定向。每个细胞通过 E-钙黏蛋白/α-连环蛋白机械转导感知到追随者的迁移，并与它们对齐。因此，方向信息在整个组织中从一个细胞传播到另一个细胞。这种由追随者引导迁移细胞的方式确保了运动的长程协调和发育稳健性。
