Yamashita Susumu, Miyagi Chiemi, Carmany-Rampey Amanda, Shimizu Takashi, Fujii Ritsuko, Schier Alexander F, Hirano Toshio
Department of Molecular Oncology (C-7), Osaka University Graduate School of Medicine, 2-2 Yamada-oka, 565-0871, Suita Osaka, Japan
Dev Cell. 2002 Mar;2(3):363-75. doi: 10.1016/s1534-5807(02)00126-0.
Vertebrate axis formation requires both the correct specification of cell fates and the coordination of gastrulation movements. We report that the zebrafish signal transducer and activator of transcription 3 (Stat3) is activated on the dorsal side by the maternal Wnt/beta-catenin pathway. Zebrafish embryos lacking Stat3 activity display abnormal cell movements during gastrulation, resulting in a mispositioned head and a shortened anterior-posterior axis, but show no defects in early cell fate specification. Time course analysis, cell tracing, and transplantation experiments revealed that Stat3 activity is required cell autonomously for the anterior migration of dorsal mesendodermal cells and non-cell autonomously for the convergence of neighboring paraxial cells. These results reveal a role for Stat3 in controlling cell movements during gastrulation.
脊椎动物的轴形成既需要细胞命运的正确特化，也需要原肠胚形成运动的协调。我们报道斑马鱼信号转导子和转录激活子3（Stat3）在背侧被母体Wnt/β-连环蛋白信号通路激活。缺乏Stat3活性的斑马鱼胚胎在原肠胚形成期间表现出异常的细胞运动，导致头部位置异常和前后轴缩短，但在早期细胞命运特化方面没有缺陷。时间进程分析、细胞追踪和移植实验表明，Stat3活性对于背侧中内胚层细胞的向前迁移是细胞自主需要的，而对于相邻轴旁细胞的汇聚是非细胞自主需要的。这些结果揭示了Stat3在控制原肠胚形成期间细胞运动中的作用。
