细胞周期对哺乳动物细胞中核苷前体的脱氧核糖核苷酸和DNA标记的依赖性影响。
Cell cycle-dependent effects on deoxyribonucleotide and DNA labeling by nucleoside precursors in mammalian cells.
作者信息
Leeds J M, Mathews C K
出版信息
Mol Cell Biol. 1987 Jan;7(1):532-4. doi: 10.1128/mcb.7.1.532-534.1987.
Abstract
dCTP pools equilibrated to equivalent specific activities in Chinese hamster ovary cells or in nuclei after incubation of cells with radiolabeled nucleosides, indicating that dCTP in nuclei does not constitute a distinct metabolic pool. In the G1 phase, [5-3H]deoxycytidine labeled dCTP to unexpectedly high specific activities. This may explain reports of replication-excluded DNA precursor pools.
摘要
在用放射性标记的核苷孵育细胞后，中国仓鼠卵巢细胞或细胞核中的dCTP池达到了等效的比活性，这表明细胞核中的dCTP并不构成一个独特的代谢池。在G1期，[5-³H]脱氧胞苷将dCTP标记到了出乎意料的高比活性。这可能解释了关于复制排除的DNA前体池的报道。
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