Kosemani Samson O, Bakare Aminat A, Adaramoye Oluwatosin A
Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, Nigeria.
Department of Biochemistry, BOWEN University, Iwo, Nigeria.
Avicenna J Phytomed. 2022 Mar-Apr;12(2):131-144. doi: 10.22038/AJP.2021.18641.
(CP) is used in Nigeria for the treatment of breast diseases. We investigated the effects of fraction from CP on 7, 12-dimethylbenz(a)anthracene (DMBA)-induced mammary gland tumors.
Female Wistar rats (40) were allotted into five equal groups. Group 1 served as control, group 2 received DMBA (50 mg/kg), groups 3 and 4 received DMBA and were treated with CP at doses of 50 and 100 mg/kg respectively, and the group 5 received DMBA and vincristine (0.5 mg/kg). DMBA was injected intraperitoneally once while vincristine and CP were given twice and thrice per week, respectively.
Administration ofDMBA caused a significant decrease in body weight gain by 52%. In addition, DMBA significantly increased organo-somatic weight of mammary gland by 4.0 folds. Also, DMBA significantly increased inflammatory and oxidative stress markers serum interleukin-1β (IL-1β), lipid peroxidation (LPO) and myeloperoxidase (MPO) by 27, 18 and 435%, respectively. Similarly, mammary NO (nitric oxide) and LPO were increased by 468 and 21%, respectively. In contrast, DMBA decreased the levels of apoptotic markers BAX, caspases 3 and 9 by 20, 15 and 18%, and mammary superoxide dismutase (SOD), catalase (CAT) and glutathione-s-peroxidase (GPx) by 45, 51 and 68%, respectively. Histology revealed gland with malignant epithelial cells and high nucleo-cytoplasm in DMBA-administered rats. Treatment with CP 100 mg/kg decreased LPO, MPO, IL-1β and NO by 28, 35, 78 and 85%, respectively, and ameliorated DMBA-induced cyto-architectural anomalies.
Fraction of CP protects mammary gland from DMBA insults via antioxidative and anti-inflammatory mechanisms.
在尼日利亚，可乐果（CP）被用于治疗乳腺疾病。我们研究了可乐果提取物对7,12-二甲基苯并(a)蒽（DMBA）诱导的乳腺肿瘤的影响。
将40只雌性Wistar大鼠平均分为五组。第1组作为对照组，第2组给予DMBA（50 mg/kg），第3组和第4组给予DMBA并分别用50和100 mg/kg的可乐果进行治疗，第5组给予DMBA和长春新碱（0.5 mg/kg）。DMBA腹腔注射一次，长春新碱和可乐果分别每周给药两次和三次。
给予DMBA导致体重增加显著下降52%。此外，DMBA使乳腺的器官-体质量显著增加4.0倍。同样，DMBA使炎症和氧化应激标志物血清白细胞介素-1β（IL-1β）、脂质过氧化（LPO）和髓过氧化物酶（MPO）分别显著增加27%、18%和435%。同样，乳腺中的一氧化氮（NO）和LPO分别增加了468%和21%。相反，DMBA使凋亡标志物BAX、半胱天冬酶3和9的水平分别降低了20%、15%和18%，乳腺超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）分别降低了45%、51%和68%。组织学检查显示，给予DMBA的大鼠乳腺中有恶性上皮细胞且核质比高。用100 mg/kg的可乐果治疗可使LPO、MPO、IL-1β和NO分别降低28%、35%、78%和85%，并改善了DMBA诱导的细胞结构异常。
可乐果提取物通过抗氧化和抗炎机制保护乳腺免受DMBA的损害。
