Department of Anesthesiology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
Department of Endocrinology and Metabolism, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
Biomed Res Int. 2022 May 16;2022:9339824. doi: 10.1155/2022/9339824. eCollection 2022.
Postoperative cognitive dysfunction (POCD) in elderly patients undergoing general anesthesia is a major problem in the aging society. Sevoflurane is the most widely applied anesthetic in clinical practice. In this study, we investigated the effects of the GLP-1 analogue liraglutide on cognitive function in aged rats anesthetized by sevoflurane. Specifically, 48 Sprague-Dawley rats were divided into the control (C) group, the liraglutide (L) group, the sevoflurane (S) group, and the sevoflurane+liraglutide (SL) group, each group with 12 rats. In the S group and the SL group, the rats were injected subcutaneously with normal saline and liraglutide after inhalation of a mixture of 3% sevoflurane and pure oxygen. In the C group and the L group, normal saline and liraglutide were injected subcutaneously into the rats after inhalation of pure oxygen. Morris Water Maze Task was applied for the detection of spatial learning and memory in rats; HE and TUNEL for staining; and western blot for quantifying Bax, Bcl-2 expression, and examining caspase-3 activity in hippocampal tissues as well as for revealing the antiapoptotic mechanism. Besides, the accumulation of inflammatory factors NF-B and IL-1 in the hippocampal tissue was quantitatively studied to reveal the anti-inflammatory mechanism. The protective effect of liraglutide on sevoflurane toxicity was the first to be confirmed in this study. Additionally, this study elucidated the mechanism of the above effect. The results of this study might be helpful to find an effective medical solution for the treatment of POCD caused by sevoflurane anesthesia.
术后认知功能障碍（POCD）是老年患者接受全身麻醉的一个主要问题。在临床实践中，七氟醚是应用最广泛的麻醉剂。在这项研究中，我们研究了 GLP-1 类似物利拉鲁肽对七氟醚麻醉老年大鼠认知功能的影响。具体来说，将 48 只 Sprague-Dawley 大鼠分为对照组（C 组）、利拉鲁肽组（L 组）、七氟醚组（S 组）和七氟醚+利拉鲁肽组（SL 组），每组 12 只大鼠。在 S 组和 SL 组中，大鼠吸入 3%七氟醚和纯氧混合气体后，皮下注射生理盐水和利拉鲁肽。在 C 组和 L 组中，大鼠吸入纯氧后皮下注射生理盐水和利拉鲁肽。应用 Morris 水迷宫任务检测大鼠的空间学习和记忆；HE 和 TUNEL 染色；Western blot 定量检测海马组织中 Bax、Bcl-2 的表达和 caspase-3 的活性，揭示抗凋亡机制；此外，还定量研究了海马组织中炎症因子 NF-B 和 IL-1 的积累，以揭示抗炎机制。本研究首次证实了利拉鲁肽对七氟醚毒性的保护作用。此外，本研究还阐明了上述作用的机制。该研究的结果可能有助于找到治疗七氟醚麻醉引起的 POCD 的有效医疗方法。
