Engineering and Technology Research Center of Shellfish Breeding in Liaoning Province, College of Fisheries and Life Science, Dalian Ocean University, Dalian, China.
Front Immunol. 2022 May 9;13:879337. doi: 10.3389/fimmu.2022.879337. eCollection 2022.
The clam is an important species in the marine aquaculture industry in China. However, in recent years, the aquaculture of has been negatively impacted by various bacterial pathogens. In this study, the transcriptome libraries of showing different levels of resistance to challenge with were constructed and RNA-seq was performed using the Illumina sequencing platform. Host immune factors were identified that responded to infection, including C-type lectin domain, glutathione S-transferase 9, lysozyme, methyltransferase FkbM domain, heat shock 70 kDa protein, Ras-like GTP-binding protein RHO, C1q, F-box and BTB/POZ domain protein zf-C2H2. Ten genes were selected and verified by RT-qPCR, and nine of the gene expression results were consistent with those of RNA-seq. The lectin gene in the phagosome pathway was expressed at a significantly higher level after infection, which might indicate the role of lectin in the immune response to . Comparing the results from resistant and nonresistant to increases our understanding of the resistant genes and key pathways related to challenge in this species. The results obtained here provide a reference for future immunological research focusing on the response of to infection.
蛤是中国海水养殖业中的一种重要物种。然而，近年来， 的养殖受到了各种细菌病原体的负面影响。在本研究中，构建了对 挑战表现出不同抗性水平的 转录组文库，并使用 Illumina 测序平台进行了 RNA-seq。鉴定了对 感染有反应的宿主免疫因子，包括 C 型凝集素结构域、谷胱甘肽 S-转移酶 9、溶菌酶、甲基转移酶 FkbM 结构域、热休克 70 kDa 蛋白、Ras 样 GTP 结合蛋白 RHO、C1q、F-box 和 BTB/POZ 结构域蛋白 zf-C2H2。选择了 10 个基因并通过 RT-qPCR 进行了验证，其中 9 个基因的表达结果与 RNA-seq 一致。吞噬体途径中的凝集素基因在 感染后表达水平显著升高，这可能表明凝集素在对 的免疫反应中发挥作用。比较 对 的抗性和非抗性结果，增加了我们对该物种对 挑战的抗性基因和关键途径的理解。本研究结果为未来针对 感染的 免疫反应的免疫研究提供了参考。
