Jiang Kunyin, Yin Zhihui, Zhang Yanming, Xu Qiaoyue, Yu Yongchao, Cong Wanlin, Yan Xiwu, Nie Hongtao
School of Fisheries and Life Science, Dalian Ocean University, 116023 Dalian, China; Engineering Research Center of Shellfish Culture and Breeding in Liaoning Province, Dalian Ocean University, 116023 Dalian, China.
Rongcheng Marine Economic Development Center, 264300 Rongcheng, China.
Genomics. 2021 May;113(3):1136-1145. doi: 10.1016/j.ygeno.2021.02.013. Epub 2021 Feb 25.
In this study, 18 MACPF genes (RpMACPF) were identified and classed into three types (Macrophage-expressed gene 1, Apextrin, and MACPF domain contain protein) based on gene structure and phylogenetic relationship in R. philippinarum. The length of RpMACPF proteins varied from 287 to 785 amino acids. The molecular weights and Theoretical PI values ranged from 3.2 kDa to 8.7 kDa and 4.7 to 8.6, respectively. RNA-seq data analysis revealed that 14 of 18 RpMACPF genes were highly expressed at the pediveliger larvae stage indicate RpMACPF might contribute to the early development and metamorphosis processes of the R. philippinarum. Besides, we found RpMACPF genes were significantly regulated by pathogen-associated molecular patterns (PAMPs) and Vibrio parahemolyticus, which indicates RpMACPF genes may play significant roles in response to invading pathogens. The results obtained in this work will provide valuable insight into the immune function of MACPF gene in R. philippinarum.
在本研究中，基于菲律宾蛤仔的基因结构和系统发育关系，鉴定出18个MACPF基因（RpMACPF），并将其分为三种类型（巨噬细胞表达基因1、Apextrin和含MACPF结构域蛋白）。RpMACPF蛋白的长度在287至785个氨基酸之间变化。分子量和理论PI值分别在3.2 kDa至8.7 kDa和4.7至8.6之间。RNA测序数据分析显示，18个RpMACPF基因中的14个在面盘幼虫阶段高表达，表明RpMACPF可能有助于菲律宾蛤仔的早期发育和变态过程。此外，我们发现RpMACPF基因受病原体相关分子模式（PAMPs）和副溶血性弧菌的显著调控，这表明RpMACPF基因可能在应对入侵病原体方面发挥重要作用。本研究获得的结果将为菲律宾蛤仔中MACPF基因的免疫功能提供有价值的见解。
