Xu Juan, Wang Yiyi, Zhang Yi, Xiong Kehui, Yan Xiaoyun, Ruan Shiyu, Wu Xueqian
State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin'an, China.
Zhejiang Provincial Key Laboratory of Characteristic Traditional Chinese Medicine Resources Protection and Innovative Utilization, Zhejiang A&F University, Lin'an, China.
Front Microbiol. 2022 May 9;13:878110. doi: 10.3389/fmicb.2022.878110. eCollection 2022.
Triterpenoids are crucial active ingredients of () with various health benefits. Yet the low biosynthesis greatly restricts their industrial applications, novel metabolic engineering strategies are needed to further enhance triterpenoids production. Transcription factors play vital roles in the metabolic regulation of terpenoids, which are still scarce to study in . Herein, a transcription factor (GenBank No. MZ436906.1) potential for metabolic regulation of triterpenes was identified for the first time. MeJA could increase triterpenoids biosynthesis, and significantly responded to MeJA induction, suggesting is a new target for triterpenoids overproduction. The regulatory effect of the newly identified target was further validated by homologous gene overexpression and silence in . It's demonstrated that overexpression of significantly increased triterpenoids accumulation and the key enzyme genes transcription in the biosynthetic pathway, while silencing it displayed the opposite effect, indicating could positively regulate the triterpenoids biosynthesis by activating the synergistic expression of key enzyme genes in the biosynthetic pathway. Consequently, was identified as a positive regulator and novel metabolic target for triterpenoids biosynthesis, it sheds new lights on the regulatory effect regulation and synthetic biology of triterpenoids.
三萜类化合物是（）的关键活性成分，具有多种健康益处。然而，其生物合成量低极大地限制了它们的工业应用，需要新的代谢工程策略来进一步提高三萜类化合物的产量。转录因子在萜类化合物的代谢调控中起着至关重要的作用，而在（）中对其研究仍然较少。在此，首次鉴定出一种对三萜类化合物具有代谢调控潜力的转录因子（GenBank登录号：MZ436906.1）。茉莉酸甲酯（MeJA）可以增加三萜类化合物的生物合成，并且（该转录因子）对MeJA诱导有显著响应，这表明（该转录因子）是三萜类化合物过量生产的一个新靶点。通过在（）中对同源基因进行过表达和沉默，进一步验证了新鉴定靶点的调控作用。结果表明，（该转录因子）的过表达显著增加了三萜类化合物的积累以及生物合成途径中关键酶基因的转录，而沉默它则表现出相反的效果，这表明（该转录因子）可以通过激活生物合成途径中关键酶基因的协同表达来正向调控三萜类化合物的生物合成。因此，（该转录因子）被鉴定为三萜类化合物生物合成的正向调节因子和新的代谢靶点，这为三萜类化合物的调控作用机制及合成生物学研究提供了新的思路。
