Du Jiang, Qiao Han, Xie Da-Long
Department of Thoracic Surgery, Chinese Medical University Affiliated No. 1 Hospital, Shenyang, Liaoning, China.
Gen Physiol Biophys. 2022 May;41(3):231-243. doi: 10.4149/gpb_2022009.
We attempted to discover the biomarker associated with metastasis and prognosis of lung adenocarcinoma. The mRNA/lncRNA expression profiles (GSE101836) were downloaded from the publicly available database, which included three highly metastatic and three weakly metastatic samples. The differentially expressed genes and lncRNAs were analyzed and survival analysis were performed based on the TCGA database. The prognosis-associated PPI network and mRNA-lncRNA coexpression network were constructed followed by the function and pathway enrichment analysis. The expression levels of key genes were validated in other datasets. Difference in gender was analyzed. Total 256 differentially expressed genes and 2 lncRNAs were found to be closely related with prognosis. PPI network was constructed with 222 nodes and 1464 edges. Two modules were divided from PPI network. Genes in module A were significantly enriched in cell cycle checkpoint, chromosome segregation, and mitotic cell cycle checkpoint. The module B was closely related with pyridine nucleotide metabolic process, nicotinamide nucleotide metabolic process and carbon metabolism. Coexpression network revealed lncRNA H19 and lncRNA SNHG12 were significant nodes. SNHG12 was closely related with GO:0006260DNA replication, GO:0055114oxidation-reduction process and hsa00010: Glycolysis/Gluconeogenesis. H19 was enriched in GO:0006555methionine metabolic process, and GO:0046655folic acid metabolic process. The expression levels of TTK and CCNB1 were confirmed in other datasets. The expression of TTK and CCNB1 was significantly higher in the male group than in the female group. TTK, CCNB1 and lncRNA SNHG12 may be the biomarker associated with metastasis and prognosis of lung adenocarcinoma.
我们试图发现与肺腺癌转移和预后相关的生物标志物。从公开数据库下载了mRNA/lncRNA表达谱（GSE101836），其中包括三个高转移样本和三个低转移样本。基于TCGA数据库对差异表达基因和lncRNAs进行分析并进行生存分析。构建预后相关的蛋白质-蛋白质相互作用（PPI）网络和mRNA-lncRNA共表达网络，随后进行功能和通路富集分析。在其他数据集中验证关键基因的表达水平。分析性别差异。共发现256个差异表达基因和2个lncRNAs与预后密切相关。构建了包含222个节点和1464条边的PPI网络。从PPI网络中划分出两个模块。模块A中的基因在细胞周期检查点、染色体分离和有丝分裂细胞周期检查点中显著富集。模块B与吡啶核苷酸代谢过程、烟酰胺核苷酸代谢过程和碳代谢密切相关。共表达网络显示lncRNA H19和lncRNA SNHG12是重要节点。SNHG12与GO:0006260DNA复制、GO:0055114氧化还原过程和hsa00010:糖酵解/糖异生密切相关。H19富集于GO:0006555甲硫氨酸代谢过程和GO:0046655叶酸代谢过程。在其他数据集中证实了TTK和CCNB1的表达水平。TTK和CCNB1在男性组中的表达明显高于女性组。TTK、CCNB1和lncRNA SNHG12可能是与肺腺癌转移和预后相关的生物标志物。
