Pukou Branch of Jiangsu People's Hospital, Nanjing City, Jiangsu Province, P. R. China.
Physiol Res. 2022 Jul 29;71(3):425-438. doi: 10.33549/physiolres.934849. Epub 2022 May 26.
100 rats were randomly divided into a sham-operated group and middle cerebral artery occlusion (MCAO) modeling groups. The sham group after surgery was observed for 14 days. After MCAO, some rats received isometric contraction training (ICT) which was as follows: an atraumatic tourniquet was placed around left or right hind limb to achieve hind limb ischemia for 5 min, followed by 5 min of reperfusion, 4 cycles for one time, once a day, and five days per week. The MCAO modeling groups included the following four groups: i) a group only received MCAO, and was observed for seven days (MCAO-7d), ii) a group only received MCAO, and was observed for 14 days (MCAO-14d), iii) a group, after MCAO, received ICT for seven days (ICT-7d), and iv) a group, after MCAO, received ICT for 14 days (ICT-14d). Brain infarct area, behavioral outcomes, the number of neurons, apoptosis, cerebral edema and cerebral water content were assessed, respectively. The mRNA expression of vascular endothelial growth factor (VEGF) was assayed with RT-PCR, and protein expression of VEGF was quantified with western blot. compared with MCAO controls, cerebral infarction, neurological deficits and neuronal apoptosis were reduced significantly in the ICT groups, while the number of neurons was increased. Moreover, the mRNA expression of VEGF and protein expression of VEGF were enhanced after 1 and 2 weeks of ICT. ICT may promote angiogenesis and neuroprotection after ischemic stroke and this new remodeling method provide a novel strategy for rehabilitation of stroke patients.
100 只大鼠被随机分为假手术组和大脑中动脉闭塞（MCAO）模型组。假手术组术后观察 14 天。MCAO 后，部分大鼠接受等长收缩训练（ICT），具体如下：在左或右后肢周围放置无创伤止血带，实现后肢缺血 5 分钟，然后再灌注 5 分钟，一次 4 个循环，每天一次，每周 5 天。MCAO 模型组包括以下四组：i）仅接受 MCAO，观察 7 天（MCAO-7d）；ii）仅接受 MCAO，观察 14 天（MCAO-14d）；iii）MCAO 后接受 ICT7 天（ICT-7d）；iv）MCAO 后接受 ICT14 天（ICT-14d）。分别评估脑梗死面积、行为学结果、神经元数量、细胞凋亡、脑水肿和脑含水量，用 RT-PCR 检测血管内皮生长因子（VEGF）的 mRNA 表达，用 Western blot 定量 VEGF 的蛋白表达。与 MCAO 对照组相比，ICT 组脑梗死面积、神经功能缺损和神经元凋亡明显减少，神经元数量增加。此外，ICT 后 1 周和 2 周时 VEGF 的 mRNA 表达和蛋白表达增强。ICT 可能促进缺血性脑卒中后的血管生成和神经保护，这种新的重塑方法为脑卒中患者的康复提供了一种新的策略。
