Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India.
J Appl Microbiol. 2022 Sep;133(3):1353-1362. doi: 10.1111/jam.15639. Epub 2022 Jun 5.
Bacteriophages are effective natural antimicrobial agents against drug-resistant pathogens. Therefore, identification and detailed characterization of bacteriophages become essential to explore their therapeutic potential. This study aims to isolate and characterize a lytic bacteriophage against drug-resistant Pseudomonas aeruginosa.
The Pseudomonas phage AIIMS-Pa-A1, isolated from the river Ganga water against drug-resistant P. aeruginosa, showed a clear lytic zone on spot assay. The phage revealed an icosahedral head (58.20 nm diameter) and a small tail (6.83 nm) under a transmission electron microscope. The growth kinetics showed an adsorption constant of 1.5 × 10 phage particles cell ml min and a latent period of approximately 15 min with the burst size of 27 phages per infected cell. The whole-genome sequencing depicted a GC-rich genome of 40.97 kb having a lysis cassette of holin, endolysin and Rz protein, with features of the family Autographiviridae. The comparative genome analysis, Ortho-average nucleotide identity value, and phylogenetic analysis indicated the novelty of the phage AIIMS-Pa-A1.
The study concludes that the Pseudomonas phage AIIMS-Pa-A1 is a novel member of the Autographiviridae family, truly lytic in nature for drug-resistant P. aeruginosa.
The Pseudomonas phage AIIMS-Pa-A1 is having promising potential for future therapeutic intervention to treat drug-resistant P. aeruginosa infections.
噬菌体是对抗耐药病原体的有效天然抗菌剂。因此，鉴定和详细描述噬菌体对于探索其治疗潜力至关重要。本研究旨在分离和鉴定一种针对耐药铜绿假单胞菌的裂解噬菌体。
从恒河水中分离出的抗药性铜绿假单胞菌噬菌体 AIIMS-Pa-A1，在点样试验中显示出明显的裂解区。透射电镜下，噬菌体呈现出二十面体头部（直径 58.20nm）和小尾巴（6.83nm）。生长动力学显示吸附常数为 1.5×10 phage 颗粒细胞 ml min，潜伏期约为 15 分钟，感染细胞的爆发量为 27 个噬菌体。全基因组测序显示，基因组为 40.97kb，富含 GC，包含溶素、内切酶和 Rz 蛋白的裂解盒，具有 Autographiviridae 家族的特征。比较基因组分析、Ortho-average nucleotide identity 值和系统发育分析表明噬菌体 AIIMS-Pa-A1 具有新颖性。
研究表明，铜绿假单胞菌噬菌体 AIIMS-Pa-A1 是 Autographiviridae 家族的新成员，对耐药铜绿假单胞菌具有真正的溶菌特性。
铜绿假单胞菌噬菌体 AIIMS-Pa-A1 具有治疗耐药铜绿假单胞菌感染的潜在治疗作用。
