Department of Pharmaceutical Biotechnology and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Department of Food and Drug Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Arch Virol. 2022 Dec 24;168(1):8. doi: 10.1007/s00705-022-05692-4.
Pseudomonas aeruginosa is an opportunistic human pathogen that can cause life-threatening nosocomial infections. The alarming increase in antibiotic resistance has led to an urgent need for alternative therapeutic approaches, such as phage therapy, which has shown promising results in many studies. In this study, P121, a new lytic Pseudomonas phage, was isolated and characterized. Whole-genome sequencing showed that it has a genome of 73,001 bp that contains 91 predicted coding sequences. No genes involved in virulence or lysogeny were found in the genome, thus making it potentially safe for therapeutic applications. Genomic and phylogenetic analysis indicated that P121 is a member of the genus Litunavirus, family Schitoviridae. The present study provides some basic information for further research on treatment of P. aeruginosa infections.
铜绿假单胞菌是一种机会性人类病原体，可导致危及生命的医院获得性感染。抗生素耐药性的惊人增加导致迫切需要替代治疗方法，例如噬菌体治疗，在许多研究中已显示出有希望的结果。在这项研究中，分离和表征了一种新的裂解性铜绿假单胞菌噬菌体 P121。全基因组测序表明，它的基因组为 73001bp，包含 91 个预测的编码序列。基因组中未发现与毒力或溶原性相关的基因，因此使其在治疗应用中具有潜在的安全性。基因组和系统发育分析表明，P121 是 Litunavirus 属、Schitoviridae 科的成员。本研究为进一步研究铜绿假单胞菌感染的治疗提供了一些基础信息。
