School of Pharmacy and Food Science, Zhuhai College of Science and Technology, Zhuhai, 519041, Guangdong, China.
Appl Biochem Biotechnol. 2022 Sep;194(9):4093-4104. doi: 10.1007/s12010-022-03976-8. Epub 2022 May 26.
Grifola frondosa is a medicinal macro-fungus with a wide range of biological activities. Polysaccharides from Grifola frondosa (PGF) play a positive role in regulating blood glucose and alleviating kidney injury. Here, we investigated the exact mechanism of action by which PGF ameliorates diabetic nephropathy. Our results showed that PGF effectively improved glucose tolerance and insulin sensitivity in streptozocin (STZ)-induced DN mice. Additionally, administration of PGF also ameliorated renal function and inflammatory response in STZ-induced DN mice. Consistent with the in vitro results, the high glucose-induced inflammatory response and apoptosis of renal tubular epithelial cells were decreased by PGF treatment. Furthermore, PGF not only suppressed the expression of TLR4, but also more effectively protected the kidney and reduced the inflammatory response when TLR4 was inhibited. All these data revealed that PGF alleviates diabetic nephropathy by blocking the TLR4/NF-κB pathway.
灰树花是一种具有广泛生物活性的药用大型真菌。灰树花子实体多糖（PGF）在调节血糖和缓解肾损伤方面发挥着积极作用。在这里，我们研究了 PGF 改善糖尿病肾病的确切作用机制。结果表明，PGF 可有效改善链脲佐菌素（STZ）诱导的糖尿病肾病小鼠的葡萄糖耐量和胰岛素敏感性。此外，PGF 还可改善 STZ 诱导的糖尿病肾病小鼠的肾功能和炎症反应。与体外结果一致，PGF 处理可降低高糖诱导的肾小管上皮细胞炎症反应和凋亡。此外，PGF 不仅抑制 TLR4 的表达，而且当 TLR4 被抑制时，更有效地保护肾脏并减轻炎症反应。所有这些数据表明，PGF 通过阻断 TLR4/NF-κB 通路来缓解糖尿病肾病。
