• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

灰树花多糖的结构表征及其对高脂饮食喂养小鼠胰岛素抵抗的影响。

Structure characterization of Grifola frondosa polysaccharide and its effect on insulin resistance in HFD-fed mice.

作者信息

Ding Yin-Yi, Lan Jinchi, Wang Yuxin, Pan Yuxiang, Song Tianyuan, Liu Shizhu, Gu Zhenyu, Ge Yujun

机构信息

National Experimental Teaching Demonstration Center of Food Engineering and Quality and Safety, Food (Edible Fungi) Processing Technology Research Center, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, 310018, China.

Zhejiang Fangge Pharmaceutical Co. Ltd, Lishui, 323800, China.

出版信息

NPJ Sci Food. 2025 Jan 8;9(1):3. doi: 10.1038/s41538-024-00359-7.

DOI:10.1038/s41538-024-00359-7
PMID:39774946
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11707143/
Abstract

Polysaccharide extracted from Grifola frondosa (GFP) was selected in this study. After preliminary separation, four factions were collected, named GFP-F1, GFP-F2, GFP-F3 and GFP-F4. GPF-F2 was further separated into two fractions, namely GFP-N1 and GFP-N2. The molecular weight of GFP-N1 and GFP-N2 was 3.323×10kDa and 10.8 kDa, respectively. GFP-N1 was composed of glucose and galactose and 1 → 3, 1 → 4, and 1 → 6 glycosidic bonds. GFP-N2 was composed of glucose, galactose and mannose and 1 → 2, 1 → 3, 1 → 4, and 1 → 6 glycosidic bonds. GFP could significantly relieve the insulin resistance induced by HFD. GFP significantly alleviated gut microbiota disturbance caused by HFD and increased the production of short-chain fatty acids, and further reduced the expression of LPS/TLR4 inflammatory pathway. GFP significantly reduced the oxidative stress induced by HFD, increased the expression of the Nrf2/ARE signaling pathway. These results indicated that GFP could be developed as a potential ingredient for the management of insulin resistance.

摘要

本研究选用了从云芝中提取的多糖（GFP）。经过初步分离，收集到四个组分，分别命名为GFP-F1、GFP-F2、GFP-F3和GFP-F4。GFP-F2进一步分离为两个组分，即GFP-N1和GFP-N2。GFP-N1和GFP-N2的分子量分别为3.323×10 kDa和10.8 kDa。GFP-N1由葡萄糖和半乳糖以及1→3、1→4和1→6糖苷键组成。GFP-N2由葡萄糖、半乳糖和甘露糖以及1→2、1→3、1→4和1→6糖苷键组成。GFP可显著缓解高脂饮食诱导的胰岛素抵抗。GFP显著减轻了高脂饮食引起的肠道微生物群紊乱，增加了短链脂肪酸的产生，并进一步降低了LPS/TLR4炎症通路的表达。GFP显著降低了高脂饮食诱导的氧化应激，增加了Nrf2/ARE信号通路的表达。这些结果表明，GFP可开发成为一种用于管理胰岛素抵抗的潜在成分。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d3c/11707143/9fff3e35b9f0/41538_2024_359_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d3c/11707143/cabcbb1030ae/41538_2024_359_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d3c/11707143/c387a1c735af/41538_2024_359_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d3c/11707143/9a2c1b806f9d/41538_2024_359_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d3c/11707143/9a0f56345cf6/41538_2024_359_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d3c/11707143/28187ea1cc43/41538_2024_359_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d3c/11707143/d6c4e465bf8e/41538_2024_359_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d3c/11707143/03423e404c3a/41538_2024_359_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d3c/11707143/e7958e35c0cf/41538_2024_359_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d3c/11707143/0b38c44234d2/41538_2024_359_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d3c/11707143/9fff3e35b9f0/41538_2024_359_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d3c/11707143/cabcbb1030ae/41538_2024_359_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d3c/11707143/c387a1c735af/41538_2024_359_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d3c/11707143/9a2c1b806f9d/41538_2024_359_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d3c/11707143/9a0f56345cf6/41538_2024_359_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d3c/11707143/28187ea1cc43/41538_2024_359_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d3c/11707143/d6c4e465bf8e/41538_2024_359_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d3c/11707143/03423e404c3a/41538_2024_359_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d3c/11707143/e7958e35c0cf/41538_2024_359_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d3c/11707143/0b38c44234d2/41538_2024_359_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d3c/11707143/9fff3e35b9f0/41538_2024_359_Fig10_HTML.jpg

相似文献

1
Structure characterization of Grifola frondosa polysaccharide and its effect on insulin resistance in HFD-fed mice.灰树花多糖的结构表征及其对高脂饮食喂养小鼠胰岛素抵抗的影响。
NPJ Sci Food. 2025 Jan 8;9(1):3. doi: 10.1038/s41538-024-00359-7.
2
Purification, Characterization and Antioxidant Activities of Enzymolysis Polysaccharide from .来自……的酶解多糖的纯化、表征及抗氧化活性
Iran J Pharm Res. 2017 Winter;16(1):347-356.
3
Structural characterization and immunomodulatory effect of a starch-like Grifola frondosa polysaccharides on cyclophosphamide-induced immunosuppression in mice.结构表征和免疫调节作用的淀粉样真菌多糖对环磷酰胺诱导的免疫抑制作用的小鼠。
Carbohydr Res. 2024 Jan;535:109011. doi: 10.1016/j.carres.2023.109011. Epub 2023 Dec 18.
4
A cold-water extracted polysaccharide-protein complex from Grifola frondosa exhibited anti-tumor activity via TLR4-NF-κB signaling activation and gut microbiota modification in H22 tumor-bearing mice.灰树花中冷水提取的多糖-蛋白复合物通过 TLR4-NF-κB 信号通路激活和 H22 荷瘤小鼠肠道微生物群的改变发挥抗肿瘤作用。
Int J Biol Macromol. 2023 Jun 1;239:124291. doi: 10.1016/j.ijbiomac.2023.124291. Epub 2023 Apr 5.
5
Preparation of a novel Grifola frondosa polysaccharide-chromium (III) complex and its hypoglycemic and hypolipidemic activities in high fat diet and streptozotocin-induced diabetic mice.一种新型灰树花多糖-三价铬（III）配合物的制备及其对高脂饮食和链脲佐菌素诱导的糖尿病小鼠的降血糖和降血脂作用。
Int J Biol Macromol. 2019 Jun 15;131:81-88. doi: 10.1016/j.ijbiomac.2019.03.042. Epub 2019 Mar 7.
6
Structural elucidation and antioxidant activity a novel Se-polysaccharide from Se-enriched Grifola frondosa.结构解析与抗氧化活性——富硒灰树花中一种新型 Se 多糖。
Carbohydr Polym. 2017 Apr 1;161:42-52. doi: 10.1016/j.carbpol.2016.12.041. Epub 2016 Dec 21.
7
The preparation of a cold-water soluble polysaccharide from Grifola frondosa and its inhibitory effects on MKN-45 cells.灰树花子实体冷水可溶多糖的制备及其对 MKN-45 细胞的抑制作用。
Glycoconj J. 2020 Aug;37(4):413-422. doi: 10.1007/s10719-020-09932-0. Epub 2020 Jun 15.
8
Grifola frondosa polysaccharides ameliorate lipid metabolic disorders and gut microbiota dysbiosis in high-fat diet fed rats.灰树花多糖可改善高脂饮食喂养大鼠的脂代谢紊乱和肠道菌群失调。
Food Funct. 2019 May 22;10(5):2560-2572. doi: 10.1039/c9fo00075e.
9
A polysaccharide from Grifola frondosa relieves insulin resistance of HepG2 cell by Akt-GSK-3 pathway.一种来自云芝的多糖通过Akt-GSK-3信号通路缓解HepG2细胞的胰岛素抵抗。
Glycoconj J. 2014 Jul;31(5):355-63. doi: 10.1007/s10719-014-9526-x. Epub 2014 Jun 8.
10
Structural properties and anti-inflammatory activity of purified polysaccharides from mushrooms ().蘑菇中纯化多糖的结构特性及抗炎活性()。
Front Nutr. 2023 Feb 7;10:1078868. doi: 10.3389/fnut.2023.1078868. eCollection 2023.

引用本文的文献

1
Mechanistic Advances in Hypoglycemic Effects of Natural Polysaccharides: Multi-Target Regulation of Glycometabolism and Gut Microbiota Crosstalk.天然多糖降血糖作用的机制进展：糖代谢的多靶点调控与肠道微生物群串扰
Molecules. 2025 Apr 29;30(9):1980. doi: 10.3390/molecules30091980.

本文引用的文献

1
Soybean-derived antihypertensive hydrolysates attenuate Ang II-induced renal damage by modulating MAPK and NF-κB signaling pathways.大豆源降压肽通过调节 MAPK 和 NF-κB 信号通路减轻 Ang II 诱导的肾损伤。
Food Funct. 2024 Mar 4;15(5):2485-2496. doi: 10.1039/d3fo05247h.
2
Glycosidic linkages of fungus polysaccharides influence the anti-inflammatory activity in mice.真菌多糖的糖苷键影响小鼠的抗炎活性。
J Adv Res. 2025 Jan;67:161-172. doi: 10.1016/j.jare.2024.01.037. Epub 2024 Feb 2.
3
Role of Oxidative Stress and Inflammation in Gestational Diabetes Mellitus.
氧化应激和炎症在妊娠期糖尿病中的作用。
Antioxidants (Basel). 2023 Sep 29;12(10):1812. doi: 10.3390/antiox12101812.
4
Modulation of PPAR-γ, SREBP-1c and inflammatory mediators by luteolin ameliorates β-cell dysfunction and renal damage in a rat model of type-2 diabetes mellitus.木樨草素通过调节 PPAR-γ、SREBP-1c 和炎症介质改善 2 型糖尿病大鼠β细胞功能障碍和肾脏损伤。
Mol Biol Rep. 2023 Nov;50(11):9129-9142. doi: 10.1007/s11033-023-08804-8. Epub 2023 Sep 26.
5
Dityrosine Aggravates Hepatic Insulin Resistance in Obese Mice by Altering Gut Microbiota and the LPS/TLR4/NF-κB Inflammatory Pathway.二酪氨酸通过改变肠道微生物群和 LPS/TLR4/NF-κB 炎症途径加剧肥胖小鼠的肝胰岛素抵抗。
Mol Nutr Food Res. 2023 Nov;67(22):e2300373. doi: 10.1002/mnfr.202300373. Epub 2023 Sep 19.
6
Effect of Oat β-Glucan on the Structure and Properties of Soybean Protein Isolate During Maillard Reaction.燕麦β-葡聚糖对美拉德反应过程中大豆分离蛋白结构与功能特性的影响。
Plant Foods Hum Nutr. 2023 Sep;78(3):552-556. doi: 10.1007/s11130-023-01092-4. Epub 2023 Aug 18.
7
Gut microbiota, intestinal permeability, and systemic inflammation: a narrative review.肠道微生物群、肠道通透性和全身炎症：叙述性综述。
Intern Emerg Med. 2024 Mar;19(2):275-293. doi: 10.1007/s11739-023-03374-w. Epub 2023 Jul 28.
8
Butyrate limits inflammatory macrophage niche in NASH.丁酸盐限制 NASH 中的炎症性巨噬细胞龛位。
Cell Death Dis. 2023 May 18;14(5):332. doi: 10.1038/s41419-023-05853-6.
9
Antioxidants attenuate mitochondrial oxidative damage through the Nrf2 pathway: A promising therapeutic strategy for stroke.抗氧化剂通过 Nrf2 通路减轻线粒体氧化损伤：脑卒中治疗的有前景策略。
J Neurosci Res. 2023 Aug;101(8):1275-1288. doi: 10.1002/jnr.25194. Epub 2023 Mar 28.
10
A Se-enriched Grifola frondosa polysaccharide induces macrophage activation by TLR4-mediated MAPK signaling pathway.富硒灰树花多糖通过 TLR4 介导的 MAPK 信号通路诱导巨噬细胞活化。
Int J Biol Macromol. 2023 May 31;238:124108. doi: 10.1016/j.ijbiomac.2023.124108. Epub 2023 Mar 21.