College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
Biopharmaceutical Lab, College of Life Science, Northeast Agricultural University, Harbin, People's Republic of China.
Environ Sci Pollut Res Int. 2022 Oct;29(48):73001-73010. doi: 10.1007/s11356-022-20734-9. Epub 2022 May 26.
Cadmium (Cd) is an environmental pollutant that can cause endocrine organ damage. To explore the effect of subacute CdCl exposure on piglet adrenal gland tissue and its mechanism based on the establishment of this model, bioinformatics, TUNEL assay, western blot (WB), and qRT-PCR methods were used to detect related indicators. The results showed that after Cd exposure, antioxidant enzymes decreased, heat shock protein increased, and miR-9-5p-gene of phosphatase and tensin homolog (PTEN) upregulates the phosphatidylinositol-3-kinase (PI3K/AKT) pathway. After this pathway was activated, the expression of the apoptosis-related factors cysteinyl aspartate-specific proteinase 3 and 9 (caspase 3 and 9), B-cell lymphoma-2-associated X (BAX) was increased sharply, and the expression of B-cell lymphoma-2 (BCL2) was significantly decreased. The changes in these indicators indicate that Cd exposure induces apoptosis and causes tissue damage in the adrenal gland of piglets. This study aims to reveal the toxic effects of CdCl in animals and will provide new ideas for the toxicology of Cd.
镉（Cd）是一种环境污染物，可导致内分泌器官损伤。为了基于该模型的建立，探索亚急性 CdCl 暴露对仔猪肾上腺组织的影响及其机制，本研究采用生物信息学、TUNEL 检测、Western blot（WB）和 qRT-PCR 方法检测相关指标。结果表明，Cd 暴露后，抗氧化酶减少，热休克蛋白增加，磷酸酶和张力蛋白同源物（PTEN）的 miR-9-5p 上调磷脂酰肌醇-3-激酶（PI3K/AKT）途径。该途径激活后，凋亡相关因子半胱氨酸天冬氨酸特异性蛋白酶 3 和 9（caspase 3 和 9）、B 细胞淋巴瘤-2 相关 X（BAX）的表达急剧增加，B 细胞淋巴瘤-2（BCL2）的表达明显降低。这些指标的变化表明，Cd 暴露诱导了仔猪肾上腺的细胞凋亡，导致组织损伤。本研究旨在揭示 CdCl 在动物中的毒性作用，为 Cd 的毒理学研究提供新的思路。
