Key Laboratory of Agro-Forestry Environmental Processes and Ecological Regulation of Hainan Province/Center for Eco-Environmental Restoration Engineering of Hainan Province/College of Ecology & Environment/State Key Laboratory of Marine Resource Utilization in South China Sea/Key Laboratory for Environmental Toxicology of Haikou, Haikou, Hainan University, Hainan, 570228, China.
Key Laboratory of Agro-Forestry Environmental Processes and Ecological Regulation of Hainan Province/Center for Eco-Environmental Restoration Engineering of Hainan Province/College of Ecology & Environment/State Key Laboratory of Marine Resource Utilization in South China Sea/Key Laboratory for Environmental Toxicology of Haikou, Haikou, Hainan University, Hainan, 570228, China; Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China.
Chemosphere. 2022 Sep;303(Pt 1):135099. doi: 10.1016/j.chemosphere.2022.135099. Epub 2022 May 23.
Microbial community were most resilient option for methane associated mitigation strategies. Biogas slurry provides plant nutrition and affects microbial community. However, little is known about the changes of the functional guilds (methanogen and methanotroph) in the geochemical context after addition biogas slurry. For this purpose, a pot experiment was conducted. Six treatment groups were included in this study, four with biogas slurry: water ratio (1:4, T02; 2:3, T04; 3:2, T06; 4:1, T08), one with a chemical fertilizer (F), and a control (CK). The effective tiller and biomass significantly increased by 1.9 times and 2.1 times in T02 relative to CK. The relative abundance of Bacteroidetes in the biogas slurry treatments was 31.5%, while that in CK was 11.4%. The dominant methanogens in CK, F and treatments were different at heading and mature stages. CK and F were hydrogenotrophs with relative abundance of 0.09% and 0.06%, and the treatment group was acetotrophs with mean value of 1.21% at heading stage. Compared with CK, the number of methanotrophs in the treatments at heading stage increased by 4.1 times, while that at mature increased by 10.3 times. The methanogenic community in the treatments may be shaped by the amount of biogas slurry applied rather than by biogeochemical processes at heading stage. Nevertheless, there may be existed synergistic interaction in the soil-microbes-rice system at mature stage. These findings may provide a better understanding of regulating soil respiration in agricultural land.
微生物群落是与甲烷相关减排策略的最具弹性选择。沼气渣提供植物营养，并影响微生物群落。然而，对于添加沼气渣后地球化学背景下功能类群（产甲烷菌和甲烷营养菌）的变化，人们知之甚少。为此，进行了盆栽试验。本研究包括六个处理组，其中四个处理组添加沼气渣：水比（1:4，T02；2:3，T04；3:2，T06；4:1，T08），一个处理组添加化肥（F），一个对照组（CK）。与 CK 相比，T02 中的有效分蘖数和生物量分别显著增加了 1.9 倍和 2.1 倍。沼气渣处理中的拟杆菌门相对丰度为 31.5%，而 CK 中的相对丰度为 11.4%。在抽穗期和成熟期，CK、F 和处理组中的优势产甲烷菌不同。CK 和 F 是氢营养型，相对丰度分别为 0.09%和 0.06%，而处理组是乙酸营养型，平均值为 1.21%。与 CK 相比，处理组在抽穗期的甲烷营养菌数量增加了 4.1 倍，而在成熟期增加了 10.3 倍。处理组中的产甲烷菌群落可能是由施加的沼气渣量而不是地球化学过程塑造的。然而，在成熟阶段，土壤-微生物-水稻系统中可能存在协同相互作用。这些发现可能为更好地了解调节农业用地土壤呼吸提供了依据。
