Department of Microbiology and Molecular Genetics, The Institute for Medical Research, Israel-Canada (IMRIC), Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel.
Singapore-HUJ Alliance for Research and Enterprise, MMID Phase II, Campus for Research Excellence and Technological Enterprise (CREATE), Singapore, Singapore.
Methods Mol Biol. 2022;2427:185-200. doi: 10.1007/978-1-0716-1971-1_16.
Group A streptococcus (GAS) necrotizing fasciitis (NF) causes high morbidity and mortality despite prompt intravenous administration of antibiotics, surgical soft-tissue debridement, and supportive treatment in the intensive care unit. Since there is no effective vaccine against GAS infections, a comprehensive understanding of NF pathogenesis is required to design more efficient treatments. To increase our understanding of NF pathogenesis, we need a reliable animal model that mirrors, at least in part, the infectious process in humans. This chapter describes a reliable murine model of human NF that mimics the histopathology observed in humans, namely the destruction of soft tissue, a paucity of infiltrating neutrophils, and the presence of many gram-positive cocci at the center of the infection.
A 组链球菌（GAS）坏死性筋膜炎（NF）尽管在重症监护病房中迅速给予静脉内抗生素、手术软组织清创和支持性治疗，但仍导致高发病率和死亡率。由于目前尚无针对 GAS 感染的有效疫苗，因此需要全面了解 NF 的发病机制，以设计更有效的治疗方法。为了增加我们对 NF 发病机制的理解，我们需要一种可靠的动物模型，该模型至少部分反映了人类的感染过程。本章描述了一种可靠的人类 NF 小鼠模型，该模型模拟了在人类中观察到的组织病理学，即软组织破坏，浸润性中性粒细胞缺乏和感染中心存在许多革兰氏阳性球菌。
