Ogunwole Eunice, Yakubu Jemimah Adoh, Giwa Vivian Tally
Department of Physiology, University of Medical Sciences, Ondo, Ondo State, Nigeria.
Department of Physiology, College of Health Sciences, Bingham University, New Karu, Nasarawa, Nigeria.
Curr Res Physiol. 2022 May 18;5:208-215. doi: 10.1016/j.crphys.2022.05.001. eCollection 2022.
Current researches aim at identifying modifiable risk factors for infertility, particularly dietary lifestyle. is one of the important vegetables in the human diet with reported valuable nutrients but has been linked with reproductive dysfunction in males. This study investigated the reproductive effects of fruit methanol extract in female Wistar rats. Dried fruit was extracted with methanol. Fifteen female Wistar rats (180-200 g) grouped into three (n = 5) received 1.0 mL/kg/day distilled water (control), 70 and 200 mg/kg/day of the extract once daily for 21 days via oral gavage. The estrous cycle was assessed using Marcondes and Papanicolaou methods. The histology of the tissues was evaluated by microscopy. Serum follicle-stimulating hormone, luteinizing hormone, and estrogen levels were measured using an enzyme-linked immunosorbent assay. Tissue antioxidant activities and malondialdehyde levels were assayed by spectrophotometry. Data were analyzed using the Analysis of variance at a significance of p < 0.05. The estrous cycle of the fruit methanol extracts treated rats showed normal cellular characteristics. Though fruit methanol extract increased the antioxidant activities, it reduced the body weight and follicle-stimulating hormone level and caused severe inflammation and fibrosis of the ovary and uterus. fruit methanol extract adversely altered the reproductive functions of female Wistar rats by disrupting the ovarian and uterine cytology and reducing hormone levels.
当前的研究旨在确定不孕症的可改变风险因素，尤其是饮食生活方式。[某种水果名称]是人类饮食中的重要蔬菜之一，含有多种有价值的营养成分，但已被认为与男性生殖功能障碍有关。本研究调查了[某种水果名称]果实甲醇提取物对雌性Wistar大鼠生殖功能的影响。将干燥的[某种水果名称]果实用甲醇提取。将15只雌性Wistar大鼠（180 - 200克）分为三组（每组n = 5），分别通过口服灌胃给予1.0毫升/千克/天的蒸馏水（对照组）、70毫克/千克/天和200毫克/千克/天的提取物，每天一次，持续2l天。使用马尔孔德斯和帕帕尼科拉乌方法评估发情周期。通过显微镜检查评估组织的组织学。使用酶联免疫吸附测定法测量血清促卵泡激素、促黄体生成素和雌激素水平。通过分光光度法测定组织抗氧化活性和丙二醛水平。使用方差分析对数据进行分析，显著性水平为p < 0.05。[某种水果名称]果实甲醇提取物处理的大鼠发情周期显示出正常的细胞特征。虽然[某种水果名称]果实甲醇提取物增加了抗氧化活性，但它降低了体重和促卵泡激素水平，并导致卵巢和子宫严重炎症和纤维化。[某种水果名称]果实甲醇提取物通过破坏卵巢和子宫细胞学并降低激素水平，对雌性Wistar大鼠的生殖功能产生了不利影响。
