College of Horticulture, Shenyang Agricultural University, Shenyang, People's Republic of China.
Key Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, Shenyang, People's Republic of China.
J Pineal Res. 2022 Sep;73(2):e12810. doi: 10.1111/jpi.12810. Epub 2022 Jun 9.
Melatonin (MT) functions in removing reactive oxygen species (ROS) and delaying plant senescence, thereby acting as an antioxidant; however, the molecular mechanism underlying the specific action of MT is unclear. Herein, we used the mutant plants carrying the MT decomposition gene melatonin 3-hydroxylase (M3H) in tomato to elucidate the specific mechanism of action of MT. SlM3H-OE accelerated senescence by decreasing the content of endogenous MT in plants. SlM3H is a senescence-related gene that positively regulates aging. MT inhibited the expression of the senescence-related gene SlCV to scavenge ROS, induced stable chloroplast structure, and delayed leaf senescence. Simultaneously, MT weakened the interaction between SlCV and SlPsbO/SlCAT3, reduced ROS production in photosystem II, and promoted ROS elimination. In conclusion, MT regulates ROS homeostasis and delays leaf aging in tomato plants through SlCV expression modulation.
褪黑素 (MT) 具有清除活性氧 (ROS) 和延缓植物衰老的功能，因此作为一种抗氧化剂；然而，MT 特定作用的分子机制尚不清楚。在这里，我们使用携带褪黑素分解基因 melatonin 3-hydroxylase (M3H) 的番茄突变体植物来阐明 MT 的具体作用机制。SlM3H-OE 通过降低植物内源 MT 的含量加速衰老。SlM3H 是一个与衰老相关的基因，正向调控衰老。MT 抑制衰老相关基因 SlCV 的表达以清除 ROS，诱导稳定的叶绿体结构，从而延缓叶片衰老。同时，MT 减弱了 SlCV 与 SlPsbO/SlCAT3 之间的相互作用，减少了光系统 II 中 ROS 的产生，并促进了 ROS 的清除。总之，MT 通过调节 SlCV 的表达来调节 ROS 平衡并延缓番茄植株叶片衰老。
