Department of Microbiology, Barkatullah University, Bhopal, M.P., 462026, India.
M S Swaminathan Research Foundation (MSSRF), Pillaiyarkuppam, Thondamanatham Post, Vazhuthavoor Road, Puducherry, 605502, India.
Curr Microbiol. 2022 May 27;79(7):206. doi: 10.1007/s00284-022-02897-3.
The present study was conducted to assess the biocontrol activity of biosurfactants obtained from Bacillus species A5F. The variables significantly influencing the production of biosurfactants under in vitro conditions were further optimized using response surface methodology. Optimal values of selected culture variables, i.e., glucose, soybean oil, and incubation time were 3.5 g l, 3.5 ml l, and 78 h, respectively, resulting in 2.14-fold enhancement in biosurfactant levels in 5 l fermentor. Identified biosurfactants had a significant effect on chlorophyll content, shoot biomass, number of pods, and seed weight. Biosurfactants also reduced the disease incidence in S. sclerotiorum infected soybean plants and showed antagonistic action against major phytopathogens by disrupting the hyphal cell wall. 16% reduction in ITS gene copy number was observed as compared to control with less non-target effect upon biosurfactant spray on foliar parts of soybean. Thus, the study confirms that biosurfactants from strain A5F can be used as a potent biocontrol agent to control sclerotium wilt on soybean plants.
本研究旨在评估从芽孢杆菌 A5F 获得的生物表面活性剂的生防活性。采用响应面法进一步优化了在体外条件下影响生物表面活性剂产生的变量。选择的培养变量的最佳值，即葡萄糖、大豆油和培养时间分别为 3.5 g/L、3.5 ml/L 和 78 h，在 5 L 发酵罐中使生物表面活性剂水平提高了 2.14 倍。鉴定出的生物表面活性剂对叶绿素含量、芽生物量、豆荚数和种子重量有显著影响。生物表面活性剂还降低了被 S. sclerotiorum 感染的大豆植物的发病率，并通过破坏菌丝细胞壁对主要植物病原菌表现出拮抗作用。与对照相比，ITS 基因拷贝数减少了 16%，而在对大豆叶片部分喷洒生物表面活性剂后，几乎没有非靶向效应。因此，该研究证实，来自菌株 A5F 的生物表面活性剂可用作控制大豆植物炭疽病的有效生防剂。
