Phytochemistry Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Metab Brain Dis. 2022 Aug;37(6):2061-2066. doi: 10.1007/s11011-022-01010-8. Epub 2022 May 27.
mTOR has been shown to be involved in the regulation of immune responses and differentiation of immune cells. This protein is a candidate molecule for unraveling the molecular mechanisms of autoimmune disorders such as multiple sclerosis (MS). We designed the current study to assess expression of MTOR, and four associated long non-coding RNAs (lncRNAs), namely SNHG1, SNHG3, SHNG5 and DANCR in the peripheral blood of patients with MS compared with healthy controls. Analysis of real-time PCR results has shown down-regulation of SNHG5 and DANCR in MS patients compared with controls. Sex of study participants had no significant effect on expression of either genes and the interaction of sex and disease on expression levels of all studied genes were insignificant. There was a significant negative correlation between expression levels of MTOR gene and disease duration. No other significant correlations were detected between genes expressions and clinical/demographic data. SNHG5 and DANCR transcript levels had AUC values of 0.88 and 0.68 in separation of patients with MS from healthy controls, respectively. Taken together, our study suggests participation of two mTOR-related lncRNAs, i.e. SNHG5 and DANCR in the pathophysiology of MS.
mTOR 已被证明参与免疫反应的调节和免疫细胞的分化。这种蛋白质是揭示多发性硬化症 (MS) 等自身免疫性疾病分子机制的候选分子。我们设计了本研究，以评估 MTOR 以及四个相关的长非编码 RNA (lncRNA)，即 SNHG1、SNHG3、SNHG5 和 DANCR 在 MS 患者与健康对照组的外周血中的表达。实时 PCR 结果分析表明，与对照组相比，MS 患者的 SNHG5 和 DANCR 表达下调。研究参与者的性别对基因表达没有显著影响，性别和疾病对所有研究基因表达水平的相互作用也不显著。MTOR 基因表达水平与疾病持续时间呈显著负相关。未检测到基因表达与临床/人口统计学数据之间的其他显著相关性。SNHG5 和 DANCR 转录本水平在将 MS 患者与健康对照组区分开来时，AUC 值分别为 0.88 和 0.68。综上所述，我们的研究表明，两种与 mTOR 相关的 lncRNA，即 SNHG5 和 DANCR，参与了 MS 的病理生理学过程。
