Institute of Research and Development, Duy Tan University, Da Nang, 550000, Vietnam.
Department of Immunology, Ophthalmology and ENT, School of Medicine, Complutense University, Madrid, Spain.
J Mol Neurosci. 2020 Oct;70(10):1533-1540. doi: 10.1007/s12031-020-01646-0. Epub 2020 Jun 23.
Recent studies have shown contribution of long non-coding RNAs (lncRNAs) in the pathogenesis of immune-related disorders including multiple sclerosis (MS). Based on the role of these transcripts in the regulation of immune response, peripheral levels of lncRNAs can reflect the level of immune activation. In the present study, we quantified expression of four lncRNAs namely SPRY4-IT1, HOXA-AS2, LINC-ROR, and MEG3 in venous blood of MS patients and controls using quantitative real-time PCR method. Relative expressions of SPRY4-IT1, HOXA-AS2, LINC-ROR, and MEG3 were significantly lower in female MS patients compared with female healthy subjects. For MEG3, this pattern of expression was also observed in male subjects. However, for other lncRNAs, no significant difference was detected between male patients and male controls. Expression of HOXA-AS2 was correlated with progression index (r = 0.36, P < 0.001). Besides, there was a significant correlation between expression of this lncRNA and expression of LINC-ROR in MS patients (r = 0.44, P < 0.0001). There was no other correlation between expression of lncRNAs and clinical data in MS patients. In control group, expressions of none of lncRNAs were correlated with age of persons. Notably, significant correlations were demonstrated between expression levels of all lncRNAs in healthy subjects with r values ranging from 0.23 to 0.42. The current investigation shows dysregulation of lncRNAs in MS patients in a sex-specific manner and warrants further studies to unravel the clinical and therapeutic implications of such dysregulation.
最近的研究表明，长非编码 RNA（lncRNA）在包括多发性硬化症（MS）在内的免疫相关疾病的发病机制中具有重要作用。基于这些转录物在免疫反应调节中的作用，lncRNA 的外周水平可以反映免疫激活的水平。在本研究中，我们使用定量实时 PCR 方法定量检测了 MS 患者和对照组静脉血中 4 种 lncRNA（SPRY4-IT1、HOXA-AS2、LINC-ROR 和 MEG3）的表达。与健康女性对照组相比，女性 MS 患者的 SPRY4-IT1、HOXA-AS2、LINC-ROR 和 MEG3 的相对表达水平显著降低。对于 MEG3，这种表达模式也在男性受试者中观察到。然而，对于其他 lncRNA，未检测到男性患者与男性对照组之间存在显著差异。HOXA-AS2 的表达与进展指数呈正相关（r=0.36，P<0.001）。此外，在 MS 患者中，该 lncRNA 的表达与 LINC-ROR 的表达呈显著正相关（r=0.44，P<0.0001）。在 MS 患者中，lncRNA 的表达与临床数据之间无其他相关性。在对照组中，lncRNA 的表达与个体年龄均无相关性。值得注意的是，健康受试者中所有 lncRNA 的表达水平之间均存在显著相关性，r 值范围为 0.23 至 0.42。本研究表明，lncRNA 在 MS 患者中存在性别特异性失调，并需要进一步研究以揭示这种失调的临床和治疗意义。
