Marouda Christina, Anagnostou Tilemahos, Savvas Ioannis, Papazoglou Lysimachos G, Psalla Dimitra
Laboratory of Pathology, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Unit of Anaesthesiology and Intensive Care, Companion Animal Clinic, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54627 Thessaloniki, Greece.
Vet Sci. 2022 Apr 22;9(5):202. doi: 10.3390/vetsci9050202.
Mast cell tumor (MCT) is a frequent cutaneous tumor in dogs, with a variable biological behavior. Studies correlate cytologic and histopathologic features of MCTs with their biological behavior, prognosis, and response to treatment. The use of preoperative opioids is common in canine patients undergoing surgical removal of these tumors. Certain opioids can induce or downregulate mast cell degranulation and influence cancer progression. The aim of the present study was to investigate whether the administration of morphine or butorphanol during surgical excision of canine cutaneous MCTs affects their cytologic and histopathologic appearance, thus influencing cytologic and histopathologic grading. This was a prospective, blinded, randomized, cohort clinical study. Forty-five dogs with cutaneous MCTs were randomly allocated into three groups according to preanaesthetic medication: dexmedetomidine combined with morphine (group M) or butorphanol (group B) or normal saline (group C). Cytologic specimens and histopathologic samples were obtained both prior to and after surgery. Samples were graded according to Kiupel's and Patnaik's systems, examined immunohistochemically for Ki-67 protein (Ki-67) and c-kit proto-oncogene product (KIT) expression, and histochemically for argyrophilic nucleolar organizing regions (AgNORs). Based on both Kiupel's and Patnaik's systems, no statistically significant differences were noted concerning the number of cases with grading discrepancies in grades allocated prior to versus after surgery among the groups. The same applied for cytological grading and immunohistochemical and histochemical evaluation. It seems that administration of morphine or butorphanol as part of the preanesthetic medication for surgical removal of canine cutaneous mast cell tumors does not influence histopathologic and cytologic grading of MCTs.
肥大细胞瘤（MCT）是犬类常见的皮肤肿瘤，其生物学行为具有多样性。研究将MCT的细胞学和组织病理学特征与其生物学行为、预后及治疗反应相关联。在接受这些肿瘤手术切除的犬类患者中，术前使用阿片类药物很常见。某些阿片类药物可诱导或下调肥大细胞脱颗粒并影响癌症进展。本研究的目的是调查在犬类皮肤MCT手术切除过程中给予吗啡或布托啡诺是否会影响其细胞学和组织病理学外观，从而影响细胞学和组织病理学分级。这是一项前瞻性、盲法、随机、队列临床研究。45只患有皮肤MCT的犬根据麻醉前用药随机分为三组：右美托咪定联合吗啡（M组）或布托啡诺（B组）或生理盐水（C组）。在手术前后均获取细胞学标本和组织病理学样本。样本根据基佩尔（Kiupel）和帕特纳伊克（Patnaik）系统进行分级，免疫组织化学检测Ki-67蛋白（Ki-67）和c-kit原癌基因产物（KIT）表达，组织化学检测嗜银核仁组织区（AgNORs）。基于基佩尔和帕特纳伊克系统，各组术前与术后分级差异病例数未见统计学显著差异。细胞学分级以及免疫组织化学和组织化学评估情况相同。作为犬类皮肤肥大细胞瘤手术切除麻醉前用药一部分的吗啡或布托啡诺给药似乎不会影响MCT的组织病理学和细胞学分级。
