Permkam Chompunut, Suriyaphol Gunnaporn, Sirisawadi Sujin, Tuntivanich Nalinee
Veterinary Bioscience Program, Department of Veterinary Anatomy, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
Biochemistry Unit, Department of Veterinary Physiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
Vet Sci. 2022 May 9;9(5):227. doi: 10.3390/vetsci9050227.
The usage of canine amniotic membrane (cAM) is mainly of interest in veterinary ophthalmology. Topical formulations of cAM could deliver the beneficial properties of cAM without the need for surgical intervention. The present study aimed to investigate biological compositions of cAM and its extracts, including their corneal wound healing efficacy. In this study, canine amniotic membrane extract (cAME) and lyophilized canine amniotic membrane extract (cAMX) were developed. Bioactive molecules related to corneal wound healing, including hepatocyte growth factor, tissue inhibitor of metalloproteinase-1 and -2, Thrombospondin-1 and Interleukin-1 receptor antagonist were studied at both gene and protein expression levels. Cell viability and wound healing assays were investigated for the possibility of cAME and cAMX as topical applications. The results demonstrated that all of the relevant genes and proteins were detected in cAM, cAME and cAMX. Both cAME and cAMX showed wound healing properties in vitro and cAME at 1.0 mg/mL concentration appeared to have the best healing efficacy. In conclusion, cAME and cAMX generated for topical use provided promising results in the healing of corneal defects.
犬羊膜（cAM）的应用主要在兽医眼科领域受到关注。cAM的局部制剂能够在无需手术干预的情况下传递cAM的有益特性。本研究旨在探究cAM及其提取物的生物学成分，包括它们对角膜伤口愈合的功效。在本研究中，制备了犬羊膜提取物（cAME）和冻干犬羊膜提取物（cAMX）。在基因和蛋白质表达水平上研究了与角膜伤口愈合相关的生物活性分子，包括肝细胞生长因子、金属蛋白酶组织抑制剂-1和-2、血小板反应蛋白-1以及白细胞介素-1受体拮抗剂。对cAME和cAMX作为局部应用的可能性进行了细胞活力和伤口愈合测定。结果表明，在cAM、cAME和cAMX中均检测到所有相关基因和蛋白质。cAME和cAMX在体外均显示出伤口愈合特性，浓度为1.0 mg/mL的cAME似乎具有最佳愈合效果。总之，制备用于局部使用的cAME和cAMX在角膜缺损愈合方面取得了有前景的结果。
