Abou Elazab Mohamed F, Nasr Nasr E, Ahmed Mohamed S, Alrashdi Barakat M, Dahran Naief, Alblihed Mohamed A, Elmahallawy Ehab Kotb
Clinical Pathology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt.
Department of Biochemistry and Clinical Biochemistry, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt.
Vet Sci. 2022 May 19;9(5):240. doi: 10.3390/vetsci9050240.
A lipopolysaccharide (LPS) is a large molecule and an outer membrane glycolipid found in Gram-negative bacteria, including (). These molecules (LPS) target acute inflammatory responses and significant physiological changes. Importantly, is considered one of the most important bacterial causes of avian colibacillosis that affect domestic turkey industry. However, little information is available about the potential influence of LPS on the biochemical parameters and histopathological changes in turkey poults. Therefore, this study aimed to evaluate the influence of bacterial lipopolysaccharide (LPS) molecules on serum biomarkers and histopathological changes in turkey poults. The birds were randomly divided into five groups, as follows: group I did not receive any inoculation; group II was inoculated with sterile saline; and groups III, IV, and V were inoculated intraperitoneally with LPS at 0.01, 0.1, and 1 mg/kg of body weight (BW), respectively. The biochemical parameters and the histopathology of different organs were examined in all birds one day post-inoculation. Our results revealed hypolipidemia, hypoglycemia, a significant decrease in uric acid, and a significant increase in serum activities of aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), and creatine kinase (CK), as well as cardiac troponin T concentrations in treated groups. Moreover, there was a significant increase in α1-, β-, and γ-globulin concentrations and a decrease in albumin and α2-globulin concentrations in group V. However, a significant increase in α2- and γ-globulin levels and a decrease in albumin levels were detected in groups III and IV. In addition, significant decreases in the albumin/globulin ratio were recorded in all LPS-treated groups. Hepatocellular and cardiac muscle necrosis, slight renal changes, and massive pulmonary inflammatory reactions were recorded. This study provides valuable information about serum biomarkers, protein fractions, and histopathological changes in turkey poults treated with LPS for further investigations of pathophysiological mechanisms in avian medicine along with biomedical research.
脂多糖（LPS）是一种大分子外膜糖脂，存在于革兰氏阴性菌中，包括（此处括号内容缺失未译）。这些分子（LPS）引发急性炎症反应和显著的生理变化。重要的是，（此处括号内容缺失未译）被认为是影响家养火鸡产业的禽大肠杆菌病的最重要细菌病因之一。然而，关于LPS对小火鸡生化参数和组织病理学变化的潜在影响，目前所知甚少。因此，本研究旨在评估细菌脂多糖（LPS）分子对小火鸡血清生物标志物和组织病理学变化的影响。将雏鸡随机分为五组，如下：第一组未接受任何接种；第二组接种无菌生理盐水；第三组、第四组和第五组分别以0.01、0.1和1mg/kg体重（BW）腹腔注射LPS。接种后一天，检测所有雏鸡的生化参数和不同器官的组织病理学。我们的结果显示，处理组出现低脂血症、低血糖、尿酸显著降低、血清天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、碱性磷酸酶（ALP）、乳酸脱氢酶（LDH）和肌酸激酶（CK）活性显著升高，以及心肌肌钙蛋白T浓度升高。此外，第五组α1-、β-和γ-球蛋白浓度显著升高，白蛋白和α2-球蛋白浓度降低。然而，第三组和第四组检测到α2-和γ-球蛋白水平显著升高，白蛋白水平降低。此外，所有LPS处理组的白蛋白/球蛋白比值均显著降低。记录到肝细胞和心肌坏死、轻微的肾脏变化以及大量的肺部炎症反应。本研究提供了关于用LPS处理小火鸡后的血清生物标志物、蛋白质组分和组织病理学变化的有价值信息，以便在禽医学病理生理机制以及生物医学研究中进行进一步研究。
