Synthesis of 5'-phosphorylated oligonucleotides on a polymer support.
作者信息
Tanaka T, Yamada Y, Tamatsukuri S, Sakata T, Ikehara M
出版信息
Nucleic Acids Symp Ser. 1986(17):85-8.
PMID:3562283
Abstract
Highly lipophilic trityloxyethylamine (TEAm) or trityloxyethylaniline (TEAn) were prepared and used for the protection of the 5'-terminal phosphate. We synthesized 5'-phosphorylated oligodeoxyribonucleotides by the solid phase synthesis. TEAm or TEAn groups enabled us to isolate the desired oligonucleotide easily by the reversed phase column due to their high lipophilicity and could be removed by the aq. AcOH treatment.
摘要
相似文献
1
Synthesis of 5'-phosphorylated oligonucleotides on a polymer support.
Nucleic Acids Symp Ser. 1986(17):85-8.
2
Synthesis of high quality phosphorothioate oligonucleotides as antisense drugs. Use of I-linker in the elimination of 3'-terminal phosphorothioate monoesters.高质量硫代磷酸酯寡核苷酸作为反义药物的合成。I-连接体在消除3'-末端硫代磷酸单酯中的应用。
Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):1421-5. doi: 10.1081/NCN-120023000.
3
[Synthesis of 5'-phosphorylated oligodeoxyribonucleotides by the H-phosphonate method].[用H-膦酸酯法合成5'-磷酸化寡脱氧核糖核苷酸]
Bioorg Khim. 1989 Apr;15(4):527-9.
4
Solid phase synthesis of oligonucleotides on a crosslinked polyacrylmorpholide support.在交联聚丙烯酰吗啉载体上进行寡核苷酸的固相合成。
Nucleic Acids Symp Ser. 1980(7):233-41.
5
Solid-phase synthesis of polynucleotides: V. Synthesis of oligodeoxyribonucleotides by the phosphomonotriazolide method.多核苷酸的固相合成：V. 用磷酸单三唑酯法合成寡脱氧核糖核苷酸
Nucleic Acids Symp Ser. 1980(7):281-91.
6
Solid-phase synthesis of 5'-phosphorylated oligodeoxynucleotides.5'-磷酸化寡脱氧核苷酸的固相合成
Nucleic Acids Symp Ser. 1985(16):161-4.
7
8
[Automated synthesis of oligodeoxyribonucleotides with terminal phosphate groups].
Bioorg Khim. 1988 Aug;14(8):1034-9.
9
The 9-fluorenylmethyloxycarbonyl group as a 5'-OH protection in oligonucleotide synthesis.
Biopolymers. 1989 May;28(5):965-73. doi: 10.1002/bip.360280505.
Zotero 插件