Department of Nuclear Medicine, Medical University of Gdansk, Gdańsk, Poland.
King's College Hospital, London, SE5 9RS UK.
Acta Biochim Pol. 2022 May 27;69(2):273-281. doi: 10.18388/abp.2020_5611.
Cancer tissue obtained by biopsy or at surgery is usually handled and stored as formalin-fixed, paraffin-embedded (FFPE) blocks. While primarily prepared for histological examination, this material represents a valuable resource that is stable and transportable, and which is ideal for examination in 3 dimensions. It has been used for DNA and protein analysis but the potential for transcriptional profiling has been little exploited: the quality of the extracted RNA is uncertain and there are few reports of its efficient use. We review the background and practicalities of this approach and describe a pilot study as a worked example in which RNA of good yield and quality could be consistently obtained from differentiated areas of adrenocortical carcinoma (ACC) tissue.
通过活检或手术获得的癌症组织通常作为福尔马林固定、石蜡包埋（FFPE）块进行处理和储存。虽然主要是为了组织学检查而准备的，但这种材料是一种稳定且可运输的有价值资源，非常适合进行三维检查。它已被用于 DNA 和蛋白质分析，但转录谱分析的潜力尚未得到充分利用：提取 RNA 的质量不确定，关于其有效利用的报道也很少。我们回顾了这种方法的背景和实用性，并描述了一项试点研究作为实例，其中可以从肾上腺皮质癌（ACC）组织的分化区域持续获得产量和质量良好的 RNA。
