School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
College of Food and Biological Engineering, Jimei University, Xiamen 361021, PR China.
Food Chem. 2022 Oct 15;391:133277. doi: 10.1016/j.foodchem.2022.133277. Epub 2022 May 21.
Aflatoxins (AFs) contaminate agricultural products in a wide range of ways during their harvesting, storage and transport. Therefore, the detection of AFs has certain practical significance. Herein, a dispersive micro solid phase extraction (D-µSPE) technology was constructed based on 1-hexyl-3-methylimidazolium hexafluorophosphate ([HMIM][PF]) fabricated ZnO nanoflowers for AFs extraction from food matrix before HPLC procedure. The key parameters affecting the extraction efficiency were studied. Under optimal experimental conditions, the method showed excellent linearity with high correlation coefficients (≥0.994). LOD and LOQ were 0.034 and 0.114 μg/kg for AFB1, 0.024 and 0.082 μg/kg for AFB2, 0.067 and 0.226 μg/kg for AFG1 and 0.025 and 0.084 μg/kg for AFG2. The recovery of actual samples spiked with analytes (at 5, 15 and 20 μg/kg) were from 93.8 to 105.1%. Overall, an accurate AFs analysis method was developed and could be applied to the determination of AFs in various food and agricultural products.
黄曲霉毒素（AFs）在农产品的收获、储存和运输过程中通过多种方式污染农产品。因此，AFs 的检测具有一定的实际意义。本文基于 1-己基-3-甲基咪唑六氟磷酸盐（[HMIM][PF6]）制备的 ZnO 纳米花，构建了一种分散微固相萃取（D-µSPE）技术，用于从食品基质中提取 AFs，然后进行 HPLC 分析。研究了影响萃取效率的关键参数。在最佳实验条件下，该方法对 AFB1、AFB2、AFG1 和 AFG2 均表现出良好的线性关系，相关系数均≥0.994。LOD 和 LOQ 分别为 0.034 和 0.114μg/kg、0.024 和 0.082μg/kg、0.067 和 0.226μg/kg、0.025 和 0.084μg/kg。实际样品中添加分析物（5、15 和 20μg/kg）的回收率为 93.8%至 105.1%。总之，建立了一种准确的 AFs 分析方法，可用于各种食品和农产品中 AFs 的测定。
