Department of Chemistry, Changzhi University, Changzhi 046011, China.
Changzhi Entry-Exit Inspection and Quarantine Bureau, Changzhi 046011, China.
Food Chem. 2019 Sep 1;291:239-244. doi: 10.1016/j.foodchem.2019.04.030. Epub 2019 Apr 8.
A novel and sensitive deep eutectic solvent-based matrix solid phase dispersion (DES-MSPD) method for the determination of aflatoxins (AFB1, AFB2, AFG1, AFG2) in various crops was established using high-performance liquid chromatography with fluorescence detection (HPLC-FLD). The DES-MSPD sample preparation procedure was optimized. Based on the optimal conditions, the intra-day and inter-day variability for AFs in all crop samples was less than 7.5%. Linearity was observed with R values (>0.994). Using the present method, HPLC-FLD gave the limits of detection (LODs) of 0.03-0.10 μg/kg and the limits of quantification (LOQs) of 0.10-0.33 μg/kg for AFs. This work represents the first attempt of using DESs as a green extraction medium for the extraction of AFs in MSPD. Compared with conventional MSPD methods, the DES-MSPD procedure looks promising as a relatively simple and low cost process to build an assay that can be used for monitoring concentrations of AFs in crops.
建立了一种基于新型、灵敏的深共晶溶剂基质固相分散(DES-MSPD)方法，结合高效液相色谱荧光检测(HPLC-FLD)，用于测定各种农作物中的黄曲霉毒素(AFB1、AFB2、AFG1、AFG2)。对 DES-MSPD 样品制备程序进行了优化。在最佳条件下，所有农作物样品中 AFs 的日内和日间变异性均小于 7.5%。线性相关系数(R 值)大于 0.994。使用本方法，HPLC-FLD 对黄曲霉毒素的检测限(LOD)为 0.03-0.10μg/kg，定量限(LOQ)为 0.10-0.33μg/kg。这是首次尝试将 DES 用作 MSPD 中提取黄曲霉毒素的绿色提取介质。与传统的 MSPD 方法相比，DES-MSPD 方法看起来很有前途，因为它是一种相对简单且低成本的过程，可以建立一种用于监测农作物中黄曲霉毒素浓度的测定方法。
