Kawamura H, Ichihara I
Prostate. 1987;10(2):153-61. doi: 10.1002/pros.2990100207.
Rat ventral prostate epithelial cells were cultured in collagen gel after collagenase digestion. The primary cultures were mainly composed of single and spherical cells. After 10 days incubation in growth medium containing insulin, transferrin, and cholera toxin, there was a 3.8-fold increase in cell numbers, aggregates of which formed three-dimensional acinus-like structures. These structures consisted of one layer of cells surrounding the lumen. The cells were joined together with a junctional complex and had microvilli on the luminal surface and secretory vacuoles in the cytoplasm facing the lumen. The ultrastructural features of the cells were not altered by growth medium containing steroids. This culture system may prove to be very useful in elucidating proliferation, organization, and differentiation of prostatic epithelial cells in relation to the extracellular matrix and stromal cells.
大鼠腹侧前列腺上皮细胞经胶原酶消化后在胶原凝胶中培养。原代培养物主要由单个球形细胞组成。在含有胰岛素、转铁蛋白和霍乱毒素的生长培养基中孵育10天后，细胞数量增加了3.8倍，其聚集体形成三维腺泡样结构。这些结构由围绕管腔的一层细胞组成。细胞通过连接复合体连接在一起，在管腔表面有微绒毛，在面向管腔的细胞质中有分泌泡。含有类固醇的生长培养基不会改变细胞的超微结构特征。该培养系统可能在阐明前列腺上皮细胞与细胞外基质和基质细胞相关的增殖、组织和分化方面非常有用。
