Tokuda Y, Toda S, Masaki Z, Sugihara H
Department of Urology, Saga Medical School, Nabeshima, Saga City, Japan.
Int J Urol. 1999 Oct;6(10):509-19. doi: 10.1046/j.1442-2042.1999.00099.x.
Prostatic epithelial cells organize functional acinus structures under epithelial extracellular matrix and epithelial-stromal cell interactions. Recently, the adipose tissue, which surrounds and even exists within the prostate, has been suggested to affect the differentiation and proliferation of some cell types. Therefore, tissue fragments, which consist mainly of epithelial and fibromuscular stromal cells, were cultured in three-dimensional collagen gel matrix culture with adipocytes.
Tissue fragments of rat dorsal prostate, including both epithelial and fibromuscular stromal components, were cultured in collagen gel with or without adipocytes. Epithelial cell differentiation was evaluated with the reconstruction of acinus-like structures and with immunohistochemistry of rat dorsal prostate-specific proteins, dorsal protein-1 and probasin. The proliferation was examined by uridine uptake.
Under coculture of the fragments and adipocytes, epithelial cells reconstructed more differentiated acinus-like structures surrounded by fibromuscular stromal cells than tissue fragment culture without adipocytes. Dorsal protein-1 and probasin expressions of epithelial cells in this coculture system were the same as in rat prostate in vivo. In the coculture, epithelial cells had a higher proliferation activity.
These results indicate that adipocytes promote proliferation and differentiation of prostatic epithelial cells. Our new culture model with adipocytes suggests the importance of cell-cell interactions, including those of epithelial cells and adipocytes.
前列腺上皮细胞在上皮细胞外基质以及上皮-基质细胞相互作用下形成功能性腺泡结构。最近，有研究表明包围甚至存在于前列腺内的脂肪组织会影响某些细胞类型的分化和增殖。因此，将主要由上皮细胞和纤维肌性基质细胞组成的组织碎片与脂肪细胞一起在三维胶原凝胶基质中培养。
将包含上皮和纤维肌性基质成分的大鼠背侧前列腺组织碎片在含有或不含有脂肪细胞的胶原凝胶中培养。通过腺泡样结构的重建以及大鼠背侧前列腺特异性蛋白背侧蛋白-1和前列腺素的免疫组织化学来评估上皮细胞分化。通过尿苷摄取检测增殖情况。
与无脂肪细胞的组织碎片培养相比，在组织碎片与脂肪细胞共培养时，上皮细胞重建出了更多由纤维肌性基质细胞包围的分化良好的腺泡样结构。在该共培养系统中，上皮细胞的背侧蛋白-1和前列腺素表达与大鼠体内前列腺中的表达相同。在共培养中，上皮细胞具有更高的增殖活性。
这些结果表明脂肪细胞促进前列腺上皮细胞的增殖和分化。我们新的含脂肪细胞的培养模型表明细胞间相互作用的重要性，包括上皮细胞与脂肪细胞之间的相互作用。
