Liang Ya, Chen Yi, Tian Ting, Zhu Shuangfen, Song Kai
Department of Otorhinolaryngology, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou, China.
Department of Otorhinolaryngology, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou, China.
Transpl Immunol. 2022 Oct;74:101631. doi: 10.1016/j.trim.2022.101631. Epub 2022 May 25.
This study aims to investigate the role of ESRRG-PERM1-CKMT2 signal axis, estrogen and genioglossal muscle contractile times in the development of Obstructive sleep apnea-hypopnea syndrome (OSAHS). This is a randomized controlled trial, and a total of 48 female rats are included and divided into following four groups: control group, sham operation group, model group and treatment group. A OSAHS rat model is established and then ESRRG, PERM1 and CKMT2 protein are detected by western blotting. Genioglossal muscle contractile times and estrogen level are also by detected. Our outcomes show that the level of ESRRG, PERM1, CKMT2 protein in control group, sham operation group, and treatment group are significantly up-regulated compared with that in model group. Genioglossal muscle contractile times and estrogen level in control group, sham operation group, and treatment group are also remarkably higher than those in model group. In addition, compared to the number of OSAHS in model group, the number of OSAHS in control group, sham operation group and treatment group is obviously decreased. We can conclude that up-regulated of ESRRG, PERM1, CKMT2 and estrogen can prevent or inhibit the development of OSAHS.
本研究旨在探讨ESRRG-PERM1-CKMT2信号轴、雌激素和颏舌肌收缩次数在阻塞性睡眠呼吸暂停低通气综合征（OSAHS）发生发展中的作用。这是一项随机对照试验，共纳入48只雌性大鼠，分为以下四组：对照组、假手术组、模型组和治疗组。建立OSAHS大鼠模型，然后通过蛋白质印迹法检测ESRRG、PERM1和CKMT2蛋白。同时检测颏舌肌收缩次数和雌激素水平。结果显示，对照组、假手术组和治疗组的ESRRG、PERM1、CKMT2蛋白水平均较模型组显著上调。对照组、假手术组和治疗组的颏舌肌收缩次数和雌激素水平也显著高于模型组。此外，与模型组的OSAHS数量相比，对照组、假手术组和治疗组的OSAHS数量明显减少。我们可以得出结论，ESRRG、PERM1、CKMT2和雌激素的上调可预防或抑制OSAHS的发生发展。
