Biological Engineering, Indian Institute of Technology Gandhinagar, Palaj, Gandhinagar, GJ, India.
Biological Engineering, Indian Institute of Technology Gandhinagar, Palaj, Gandhinagar, GJ, India; Center for Biomedical Engineering, Indian Institute of Technology Gandhinagar, Palaj, Gandhinagar, GJ, India.
Methods Cell Biol. 2022;169:323-346. doi: 10.1016/bs.mcb.2022.01.002. Epub 2022 Mar 22.
From being genetic material to being exploited as an intelligent biomaterial, DNA has traveled a lot of scientific space of research and innovation. DNA can assemble into macromolecular polymeric networks based on sequences or by physically cross-linking their bulky lengthy strands. DNA is a polyanionic, hydrophilic, and polyelectrolytic natural biomaterial that can absorb large amounts of water mostly via H-bond interactions. The ability of DNA to attract water enables it form DNA-based hydrogels. DNA hydrogels offer many desirable qualities, making them an ideal choice as a desirable biomaterial for diverse applications. DNA Hydrogels show biodegradability, biocompatibility, modularity, non-toxicity, hydrophilicity, self-healing ability, and the ability to probe, program, and reprogram diverse biological systems. This chapter focuses on pure DNA-based hydrogels, their principles, and synthesis methods. We outlay various characterization tools and techniques followed by their biological applications and brief conclusion about their future employability for diverse biomedical applications.
从遗传物质到被用作智能生物材料，DNA 经历了大量的科学研究和创新。DNA 可以根据序列组装成大分子聚合网络，或者通过物理交联其庞大的长链。DNA 是一种带负电荷的、亲水性的、聚电解质的天然生物材料，主要通过氢键相互作用可以吸收大量的水。DNA 吸引水的能力使其能够形成基于 DNA 的水凝胶。DNA 水凝胶具有许多理想的特性，使其成为各种应用中理想的生物材料。DNA 水凝胶具有可生物降解性、生物相容性、可修饰性、无毒性、亲水性、自修复能力以及探测、编程和重新编程各种生物系统的能力。本章重点介绍纯基于 DNA 的水凝胶及其原理和合成方法。我们列出了各种表征工具和技术，随后介绍了它们的生物学应用，并简要总结了它们在未来用于各种生物医学应用的可利用性。
