Department of Environmental Engineering, College of Environmental and Resource Science, Zhejiang University, Hangzhou 310058, China; MOE Key Laboratory of Environmental Remediation and Ecological Health, College of Environmental and Resource Science, Zhejiang University, Hangzhou 310058, China.
Department of Environmental Engineering, College of Environmental and Resource Science, Zhejiang University, Hangzhou 310058, China.
J Environ Sci (China). 2022 Oct;120:53-62. doi: 10.1016/j.jes.2021.12.045. Epub 2022 Jan 13.
Penicillium oxalicum SL2 (SL2) is a previously screened Pb-tolerant fungus that can promote crops growth. The relationship between SL2 colonization and Pb immobilization was studied to provide a theoretical basis for microbial remediation of Pb-contaminated paddy soil. In this study, green fluorescent protein (GFP) labeled SL2 was inoculated into different Pb-contaminated paddy soils (S1-S6). The Pb extracted from the soil by HNO, EDTA and CaCl were used to characterize the available Pb. The results showed that the colonization of SL2 was divided into lag phase (0-7 days), growth phase (7-30 days), and mortality phase (30-90 days). SL2 colonized well in sandy soils rich in clay and total phosphorus with initial pH of 4.5-7.0. In addition, SL2 increased soil pH and decreased soil Eh, which was beneficial to immobilize Pb. In different soils, the highest percentages of CaCl-Pb, EDTA-Pb, and HNO-Pb immobilized by SL2 were 34.34%-40.53%, 17.05%-20.11%, and 7.39%-15.62%, respectively. Pearson correlation analysis showed that the percentages of CaCl-Pb and EDTA-Pb immobilized by SL2 were significantly positively correlated with the number of SL2 during the growth phase. SL2 mainly immobilized Pb in the growth phase and a higher peak number of SL2 was beneficial to the immobilization of Pb.
草酸青霉 SL2（SL2）是一种先前筛选出的耐铅真菌，能促进作物生长。本研究旨在探讨 SL2 定殖与 Pb 固定化之间的关系，为微生物修复 Pb 污染稻田土壤提供理论依据。本研究将绿色荧光蛋白（GFP）标记的 SL2 接种到不同 Pb 污染稻田土壤（S1-S6）中。采用 HNO3、EDTA 和 CaCl2 提取土壤中的 Pb，用以表征有效态 Pb。结果表明，SL2 的定殖可分为延滞期（0-7 天）、生长期（7-30 天）和死亡期（30-90 天）。SL2 在富含粘土和总磷、初始 pH 值为 4.5-7.0 的沙壤土中定殖良好。此外，SL2 增加了土壤 pH 值，降低了土壤 Eh 值，有利于 Pb 的固定化。在不同土壤中，SL2 固定的 CaCl2-Pb、EDTA-Pb 和 HNO3-Pb 最高百分比分别为 34.34%-40.53%、17.05%-20.11%和 7.39%-15.62%。Pearson 相关性分析表明，SL2 固定的 CaCl2-Pb 和 EDTA-Pb 百分比与生长阶段 SL2 的数量呈显著正相关。SL2 主要在生长期固定 Pb，较高的 SL2 峰值数量有利于 Pb 的固定化。
